Molecular characterization of VIM-producing Klebsiella pneumoniae from Scandinavia reveals genetic relatedness with international clonal complexes encoding transferable multidrug resistance

Samuelsen, O., Toleman, M. A., Hasseltvedt, V., Fuursted, K., Leegaard, T. M., Walsh, T. R., Sundsfjord, A. and Giske, C. G. (2011) Molecular characterization of VIM-producing Klebsiella pneumoniae from Scandinavia reveals genetic relatedness with international clonal complexes encoding transferable multidrug resistance. Clinical Microbiology and Infection, 17 12: 1811-1816. doi:10.1111/j.1469-0691.2011.03532.x

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Author Samuelsen, O.
Toleman, M. A.
Hasseltvedt, V.
Fuursted, K.
Leegaard, T. M.
Walsh, T. R.
Sundsfjord, A.
Giske, C. G.
Title Molecular characterization of VIM-producing Klebsiella pneumoniae from Scandinavia reveals genetic relatedness with international clonal complexes encoding transferable multidrug resistance
Formatted title
Molecular characterization of VIM-producing Klebsiella pneumoniae from Scandinavia reveals genetic relatedness with international clonal complexes encoding transferable multidrug resistance
Journal name Clinical Microbiology and Infection   Check publisher's open access policy
ISSN 1469-0691
1198-743X
Publication date 2011-12
Sub-type Article (original research)
DOI 10.1111/j.1469-0691.2011.03532.x
Volume 17
Issue 12
Start page 1811
End page 1816
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
VIM-producing Klebsiella pneumoniae (VPKP) has been identified as a source of hospital outbreaks and is prevalent particularly in the Mediterranean region. In this study we have characterized eight VPKP isolates identified in Scandinavia during 2005–2008. With the exception of one isolate, all were from patients with recent history of hospitalization abroad (Greece, n = 6; Turkey, n = 1). Multilocus sequence typing (MLST) resulted in five sequence types (STs), ST36 (n = 1), ST147 (n = 4), ST272 (n = 1), ST273 (n = 1) and ST383 (n = 1), which except for ST272 were part of putative international clonal complexes. All were multidrug resistant due to the presence of other resistance determinants, including extended-spectrum β-lactamases (CTX-M-3, SHV-5 and SHV-12), 16S rRNA methylases (ArmA) and plasmid-mediated quinolone resistance determinants (QnrS). One isolate harboured a novel VIM-variant (VIM-26) while VIM-1 and VIM-19 were detected in six and one isolate, respectively. Two different genetic structures surrounding the blaVIM gene were identified in four isolates. In two isolates blaVIM-1 and blaVIM-26 were located in an integron similar to In-e541 (intI1;blaVIM-1/-26;aacA7; dhfrI;aadA1;3′CS) while in the other two isolates blaVIM-1 was located in an integron lacking 3′CS but with an IS26 element in the 3′end (intI1;blaVIM-1;aac(6′)-Ib;IS26), as identified in the IncN plasmid pKOX105. The blaVIM-genes were located on transferable plasmids ranging from ∼40 to ∼240 kb and associated with Tn21 in four isolates. PCR-based replicon typing indicated association of blaVIM with IncN (n = 3) and A/C (n = 1) broad-host-range plasmids but also with unknown replicons (n = 4). In conclusion, Scandinavian VPKP is associated with importation and genetically related to international clones encoding transferable plasmid-mediated multidrug resistance.
Keyword Integrons
Klebsiella pneumoniae
Metallo-beta-lactamase
MLST
Molecular epidemiology
Plasmid-replicon typing
Scandinavia
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Non HERDC
 
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Citation counts: TR Web of Science Citation Count  Cited 39 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 31 Jan 2012, 10:25:42 EST by Roheen Gill on behalf of UQ Centre for Clinical Research