Prevalence of SXT/R391-like integrative and conjugative elements carrying bla(CMY-2) in Proteus mirabilis

Mata, Caterina, Navarro, Ferran, Miro, Elisenda, Walsh, Timothy R., Mirelis, Beatriz and Toleman, Mark (2011) Prevalence of SXT/R391-like integrative and conjugative elements carrying bla(CMY-2) in Proteus mirabilis. Journal of Antimicrobial Chemotherapy, 66 10: 2266-2270. doi:10.1093/jac/dkr286

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Author Mata, Caterina
Navarro, Ferran
Miro, Elisenda
Walsh, Timothy R.
Mirelis, Beatriz
Toleman, Mark
Title Prevalence of SXT/R391-like integrative and conjugative elements carrying bla(CMY-2) in Proteus mirabilis
Formatted title
Prevalence of SXT/R391-like integrative and conjugative elements carrying blaCMY-2 in Proteus mirabilis
Journal name Journal of Antimicrobial Chemotherapy   Check publisher's open access policy
ISSN 1460-2091
0305-7453
Publication date 2011-10
Sub-type Article (original research)
DOI 10.1093/jac/dkr286
Volume 66
Issue 10
Start page 2266
End page 2270
Total pages 5
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2012
Language eng
Formatted abstract
Objectives: To characterize the vectors involved in the dissemination of blaCMY-2 genes in clinical isolates of Proteus mirabilis collected between 1999 and 2007.

Methods: Plasmid analysis of 19 P. mirabilis carrying ampC genes was performed by PCR-based replicon typing, S1-PFGE and Southern hybridization with ampC and replicon probes. Isolates that could not be characterized were examined for the presence of SXT/R391-like elements. To demonstrate the involvement of these elements in the dissemination of blaCMY-2, we performed a PCR amplification of the integrase (int) and toxin/antitoxin (TA) genes from SXT/R391-like integrative conjugative elements (ICEs). Later on, I-Ceu-I PFGE gels and hybridization with blaCMY-2, int and prfC probes were performed. The genetic organization of blaCMY-2 was also studied.

Results: ampC genes were located on large conjugative plasmids in 11 of the 19 (58%) P. mirabilis studied. However, in eight of these isolates a plasmid was not involved in the mobilization of ampC genes. I-Ceu-I PFGE and hybridization analyses revealed that blaCMY-2 were chromosomally located in these eight P. mirabilis isolates. The genetic organization of blaCMY-2 and hybridization analyses revealed that blaCMY-2 was carried by an ICE almost identical to ICEPmiJpan1 in seven out of these eight isolates.

Conclusions: The prevalence of ICEs carrying blaCMY-2 was surprisingly high [37% (7 out of 19)]. This is the first study giving prevalence data on ICEs carrying blaCMY-2 genes. These results suggest the need to study these mobile genetic elements in the context of dissemination of acquired AmpC β-lactamases and also of other β-lactamases, such as extended- spectrum β-lactamases and carbapenemases.  
Keyword mobile genetic elements
AmpC beta-lactamases
Enterobacteriaceae
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Non HERDC
 
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 31 Jan 2012, 10:09:44 EST by Roheen Gill on behalf of UQ Centre for Clinical Research