Characterization of IncA/C conjugative plasmid harboring bla (TEM-52) and bla (CTX-M-15) extended-spectrum beta-lactamases in clinical isolates of Escherichia coli in Tunisia

Chouchani, C., El Salabi, A., Marrakchi, R., Ferchichi, L. and Walsh, T. R. (2012) Characterization of IncA/C conjugative plasmid harboring bla (TEM-52) and bla (CTX-M-15) extended-spectrum beta-lactamases in clinical isolates of Escherichia coli in Tunisia. European Journal of Clinical Microbiology and Infectious Diseases, 31 6: 1081-1087. doi:10.1007/s10096-011-1410-z


Author Chouchani, C.
El Salabi, A.
Marrakchi, R.
Ferchichi, L.
Walsh, T. R.
Title Characterization of IncA/C conjugative plasmid harboring bla (TEM-52) and bla (CTX-M-15) extended-spectrum beta-lactamases in clinical isolates of Escherichia coli in Tunisia
Formatted title
Characterization of IncA/C conjugative plasmid harboring blaTEM-52 and blaCTX-M-15 extended-spectrum β-lactamases in clinical isolates of Escherichia coli in Tunisia 
Journal name European Journal of Clinical Microbiology and Infectious Diseases   Check publisher's open access policy
ISSN 0934-9723
1435-4373
Publication date 2012-06-01
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s10096-011-1410-z
Volume 31
Issue 6
Start page 1081
End page 1087
Total pages 7
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2012
Language eng
Formatted abstract
To characterize the extended-spectrum β-lactamases (ESBLs) as well as their genetic environment in different isolates of Escherichia coli from patients with repeated urinary tract infections, large multidrug resistance (MDR) plasmids have been found. Definitive evidence for the presence of an A/C incompatibility complex (IncA/C) plasmid in the MDR isolates was provided by the probing of plasmids extracted from the clinical isolates. Conjugation experiments showed that bla genes were transferred by conjugation from the ten E. coli clinical isolates to E. coli XL1-Blue recipient. A comparative restriction fragment length polymorphism (RFLP) analysis of these plasmids showed that they are genetically similar, while the overall similarity of these plasmids supports the likelihood of recent movements among these E. coli isolates. Polymerase chain reaction (PCR) amplification and sequencing of the amplicons showed  that the IncA/C plasmids harbor two ESBLs, identified as TEM-52 and CTX-M-15. Analysis of the plasmid DNA surrounding the blaCTX-M-15 gene in the clinical isolates under study revealed a partially truncated fragment of ISEcp1 tnpA transposase. This result indicates the variety of genetic events that have enabled associations between ISEcp1 sequences and blaCTX-M-15 genes in these clinical isolates.  
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 22 September 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
 
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Created: Tue, 31 Jan 2012, 19:25:59 EST by Roheen Gill on behalf of UQ Centre for Clinical Research