First report of mefA and msrA/msrB multidrug efflux pumps associated with bla(TEM-1) beta-lactamase in Enterococcus faecalis

Chouchani, Chedly, Salabi, Allaaeddin El, Marrakchi, Rim, Ferchichi, Leila and Walsh, Timothy R. (2012) First report of mefA and msrA/msrB multidrug efflux pumps associated with bla(TEM-1) beta-lactamase in Enterococcus faecalis. International Journal of Infectious Diseases, 16 2: 104-109. doi:10.1016/j.ijid.2011.09.024


Author Chouchani, Chedly
Salabi, Allaaeddin El
Marrakchi, Rim
Ferchichi, Leila
Walsh, Timothy R.
Title First report of mefA and msrA/msrB multidrug efflux pumps associated with bla(TEM-1) beta-lactamase in Enterococcus faecalis
Journal name International Journal of Infectious Diseases   Check publisher's open access policy
ISSN 1201-9712
1878-3511
Publication date 2012-02
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.ijid.2011.09.024
Open Access Status DOI
Volume 16
Issue 2
Start page 104
End page 109
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract
Objectives: Enterococcus faecalis is thought to possess a great deal of intrinsic resistance to several antimicrobial agents. In this study we identified ampicillin- and erythromycin-resistant clinical isolates of E. faecalis and sought to identify the resistance mechanisms among these isolates.

Methods: Twelve isolates of E. faecalis were collected from 12 different patients. Identification of the isolates and their susceptibility patterns were determined using the Phoenix automated phenotypic identification criteria. PCR amplification and sequencing were used to detect b- actamase production. Colony blotting was performed in order to screen for multidrug efflux pump production. Extraction and N-terminal sequencing of the multidrug efflux pumps was carried out.

Results: The E. faecalis isolates showed high resistance to erythromycin and ampicillin, with minimum inhibitory concentrations of >16 mg/ml. PCR amplification and sequencing showed that isolates produced TEM-1 b- actamase. Colony blotting showed that these isolates harbored multidrug efflux pump genes. Multidrug efflux pump extraction, purification, and sequencing showed the distribution of mefA and msrA/msrB efflux pumps.

Conclusion: Two resistance mechanisms among E. faecalis are described – the production of TEM blactamase and mefA and msrA/msrB efflux pumps. These results are of great interest because this is the first report of the co-existence of these resistance mechanisms among E. faecalis strains.  
Keyword Enterococcus faecalis
mefA
msrA/msrB
Efflux pump
TEM-1 b-lactamase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
 
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Created: Tue, 31 Jan 2012, 08:59:47 EST by Roheen Gill on behalf of UQ Centre for Clinical Research