Phasevarion mediated epigenetic gene regulation in Helicobacter pylori

Srikhanta, Yogitha N., Gorrell, Rebecca J., Steen, Jason A., Gawthorne, Jayde A., Kwok, Terry, Grimmond, Sean M., Robins-Browne, Roy M. and Jennings, Michael P. (2011) Phasevarion mediated epigenetic gene regulation in Helicobacter pylori. PLoS One, 6 12: e27569.1-e27569.9. doi:10.1371/journal.pone.0027569

Author Srikhanta, Yogitha N.
Gorrell, Rebecca J.
Steen, Jason A.
Gawthorne, Jayde A.
Kwok, Terry
Grimmond, Sean M.
Robins-Browne, Roy M.
Jennings, Michael P.
Title Phasevarion mediated epigenetic gene regulation in Helicobacter pylori
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-12-05
Sub-type Article (original research)
DOI 10.1371/journal.pone.0027569
Open Access Status DOI
Volume 6
Issue 12
Start page e27569.1
End page e27569.9
Total pages 9
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2012
Language eng
Formatted abstract
Many host-adapted bacterial pathogens contain DNA methyltransferases (mod genes) that are subject to phase-variable expression (high-frequency reversible ON/OFF switching of gene expression). In Haemophilus influenzae and pathogenic Neisseria, the random switching of the modA gene, associated with a phase-variable type III restriction modification (R-M) system, controls expression of a phase-variable regulon of genes (a ‘‘phasevarion’’), via differential methylation of the genome in the modA ON and OFF states. Phase-variable type III R-M systems are also found in Helicobacter pylori, suggesting that phasevarions may also exist in this key human pathogen. Phylogenetic studies on the phase-variable type III modH gene revealed that there are 17 distinct alleles in H. pylori, which differ only in their DNA recognition domain. One of the most commonly found alleles was modH5 (16% of isolates). Microarray analysis comparing the wild-type P12ΔmodH5 ON strain to a P12ΔmodH5 mutant revealed that six genes were either up- or down-regulated, and some were virulenceassociated. These included flaA, which encodes a flagella protein important in motility and hopG, an outer membrane protein essential for colonization and associated with gastric cancer. This study provides the first evidence of this epigenetic mechanism of gene expression in H. pylori. Characterisation of H. pylori modH phasevarions to define stable immunological targets will be essential for vaccine development and may also contribute to understanding H. pylori pathogenesis.  
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 30 Jan 2012, 15:42:30 EST by Susan Allen on behalf of Institute for Molecular Bioscience