Caveolin-1 orchestrates the balance between glucose and lipid-dependent energy metabolism: Implications for liver regeneration

Fernandez Rojo, Manuel Alejandro, Restall, Christina, Ferguson, Charles, Martel, Nick, Martin, Sally, Bosch, Marta, Kassan, Adam, Leong, Gary M., Martin, Sheree D., McGee, Sean L., Muscat, George E., Anderson, Robin L., Enrich, Carlos, Pol, Albert and Parton, Robert G. (2012) Caveolin-1 orchestrates the balance between glucose and lipid-dependent energy metabolism: Implications for liver regeneration. Hepatology, 55 5: 1574-1584. doi:10.1002/hep.24810

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Author Fernandez Rojo, Manuel Alejandro
Restall, Christina
Ferguson, Charles
Martel, Nick
Martin, Sally
Bosch, Marta
Kassan, Adam
Leong, Gary M.
Martin, Sheree D.
McGee, Sean L.
Muscat, George E.
Anderson, Robin L.
Enrich, Carlos
Pol, Albert
Parton, Robert G.
Total Author Count Override 16
Title Caveolin-1 orchestrates the balance between glucose and lipid-dependent energy metabolism: Implications for liver regeneration
Journal name Hepatology   Check publisher's open access policy
ISSN 0270-9139
1527-3350
Publication date 2012-05
Year available 2011
Sub-type Article (original research)
DOI 10.1002/hep.24810
Volume 55
Issue 5
Start page 1574
End page 1584
Total pages 11
Place of publication Hoboken, NJ, U.S.A.
Publisher John Wiley & Sons
Collection year 2013
Language eng
Formatted abstract
To better understand the contribution of the role of caveolin-1 (CAV1) in liver physiology during liver regeneration and hepatic steatosis, we have generated CAV1 null (CAV1-/-) mouse with a pure genetic background. Study of these mice in comparison with another two different CAV1-/- mouse strains demonstrates that: i) upon regular physiological conditions expression of CAV1 in mouse tissues is necessary to guarantee an efficient progression of liver regeneration and mice survival after partial hepatectomy. ii) Expression of CAV1 in mice is required for efficient hepatic lipid storage during physiological and pathological conditions of hepatic steatosis and iii) Under these conditions CAV1 accumulates in the lipid droplet (LD) fraction in wild-type mouse hepatocytes. Interestingly, our comparative analysis also demonstrates that the absence of CAV1 in mouse tissues can be compensated by the development of a carbohydrate-dependent anabolic adaptation that in the case of the liver determines the ability of hepatocytes to undergo liver regeneration. These results were supported by extracellular flux analysis of cellular glycolytic metabolism in CAV1-knock-down AML12 hepatocytes suggesting cell autonomous effects of CAV1 loss in hepatic glycolysis.

Conclusion: Here we demonstrate the extreme importance of the expression of CAV1 in mouse tissues for the metabolism and cell biology of hepatocytes under physiological and pathological conditions.
Keyword CAV1
Hepatocytes
Glycolysis
Lipid metabolism
Lipid droplets
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Accepted manuscript online: 22 NOV 2011. © 2011 American Association for the Study of Liver Diseases.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 24 Jan 2012, 14:20:59 EST by Susan Allen on behalf of Institute for Molecular Bioscience