Characterisation of a cell wall-anchored protein of Staphylococcus saprophyticus associated with linoleic acid resistance

King, Nathan P, Sakinc, Turkan, Ben Zakour, Nouri L, Totsika, Makrina, Heras, Begona, Simerska, Pavla, Shepherd, Mark, Gatermann, Soren G, Beatson, Scott A and Schembri, Mark A (2012) Characterisation of a cell wall-anchored protein of Staphylococcus saprophyticus associated with linoleic acid resistance. BMC Microbiology, 12 1: 8.1-8.25. doi:10.1186/1471-2180-12-8


Author King, Nathan P
Sakinc, Turkan
Ben Zakour, Nouri L
Totsika, Makrina
Heras, Begona
Simerska, Pavla
Shepherd, Mark
Gatermann, Soren G
Beatson, Scott A
Schembri, Mark A
Title Characterisation of a cell wall-anchored protein of Staphylococcus saprophyticus associated with linoleic acid resistance
Journal name BMC Microbiology   Check publisher's open access policy
ISSN 1471-2180
Publication date 2012-01-15
Sub-type Article (original research)
DOI 10.1186/1471-2180-12-8
Open Access Status DOI
Volume 12
Issue 1
Start page 8.1
End page 8.25
Total pages 25
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2013
Language eng
Formatted abstract
Background
The Gram-positive bacterium Staphylococcus saprophyticus is the second most frequent causative agent of community-acquired urinary tract infections (UTI), accounting for up to 20% of cases. A common feature of staphylococci is colonisation of the human skin. This involves survival against innate immune defenses including antibacterial unsaturated free fatty acids such as linoleic acid which act by disrupting bacterial cell membranes. Indeed, S. saprophyticus UTI is usually preceded by perineal skin colonisation.

Results
In this study we identified a previously undescribed 73.5 kDa cell wall-anchored protein of S. saprophyticus, encoded on plasmid pSSAP2 of strain MS1146, which we termed S. saprophyticus surface protein F (SssF). The sssF gene is highly prevalent in S. saprophyticus clinical isolates and we demonstrate that the SssF protein is expressed at the cell surface. However, unlike all other characterised cell wall-anchored proteins of S. saprophyticus, we were unable to demonstrate a role for SssF in adhesion. SssF shares moderate sequence identity to a surface protein of Staphylococcus aureus (SasF) recently shown to be an important mediator of linoleic acid resistance. Using a heterologous complementation approach in a S. aureus sasF null genetic background, we demonstrate that SssF is associated with resistance to linoleic acid. We also show that S. saprophyticus strains lacking sssF are more sensitive to linoleic acid than those that possess it. Every staphylococcal genome sequenced to date encodes SssF and SasF homologues. Proteins in this family share similar predicted secondary structures consisting almost exclusively of α-helices in a probable coiled-coil formation.

Conclusions
Our data indicate that SssF is a newly described and highly prevalent surface-localised protein of S. saprophyticus that contributes to resistance against the antibacterial effects of linoleic acid. SssF is a member of a protein family widely disseminated throughout the staphylococci.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 23 Jan 2012, 08:32:35 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences