Time-course global expression profiles of chlamydomonas reinhardtii during photo-biological H2 production

Nguyen, Anh Vu, Toepel, Joerg, Burgess, Steven, Uhmeyer, Andreas, Blifernez, Olga, Doebbe. Anja, Hankamer, Ben, Nixon, Peter, Wobbe, Lutz and Kruse, Olaf (2011) Time-course global expression profiles of chlamydomonas reinhardtii during photo-biological H2 production. PLoS One, 6 12: e29364.1-e29364.15. doi:10.1371/journal.pone.0029364

Author Nguyen, Anh Vu
Toepel, Joerg
Burgess, Steven
Uhmeyer, Andreas
Blifernez, Olga
Doebbe. Anja
Hankamer, Ben
Nixon, Peter
Wobbe, Lutz
Kruse, Olaf
Title Time-course global expression profiles of chlamydomonas reinhardtii during photo-biological H2 production
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-12
Sub-type Article (original research)
DOI 10.1371/journal.pone.0029364
Open Access Status DOI
Volume 6
Issue 12
Start page e29364.1
End page e29364.15
Total pages 15
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2012
Language eng
Formatted abstract
We used a microarray study in order to compare the time course expression profiles of two Chlamydomonas reinhardtii strains, namely the high H2 producing mutant stm6glc4 and its parental WT strain during H2 production induced by sulfur starvation. Major cellular reorganizations in photosynthetic apparatus, sulfur and carbon metabolism upon H2 production were confirmed as common to both strains. More importantly, our results pointed out factors which lead to the higher H2 production in the mutant including a higher starch accumulation in the aerobic phase and a lower competition between the H2ase pathway and alternative electron sinks within the H2 production phase. Key candidate genes of interest with differential expression pattern include LHCSR3, essential for efficient energy quenching (qE). The reduced LHCSR3 protein expression in mutant stm6glc4 could be closely related to the high-light sensitive phenotype. H2 measurements carried out with the LHCSR3 knock-out mutant npq4 however clearly demonstrated that a complete loss of this protein has almost no impact on H2 yields under moderate light conditions. The nuclear gene disrupted in the high H2 producing mutant stm6glc4 encodes for the mitochondrial transcription termination factor (mTERF) MOC1, whose expression strongly increases during –S-induced H2 production in WT strains. Studies under phototrophic high-light conditions demonstrated that the presence of functional MOC1 is a prerequisite for proper LHCSR3 expression. Furthermore knock-down of MOC1 in a WT strain was shown to improve the total H2 yield significantly suggesting that this strategy could be applied to further enhance H2 production in other strains already displaying a high H2 production capacity. By combining our array data with previously published metabolomics data we can now explain some of the phenotypic characteristics which lead to an elevated H2 production in stm6glc4.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 16 Jan 2012, 15:03:06 EST by Susan Allen on behalf of Institute for Molecular Bioscience