Engineering cyclic peptide toxins

Clark, Richard J. and Craik, David J. (2012) Engineering cyclic peptide toxins. Methods in Enzymology, 503 57-74. doi:10.1016/B978-0-12-396962-0.00003-3

Author Clark, Richard J.
Craik, David J.
Title Engineering cyclic peptide toxins
Journal name Methods in Enzymology   Check publisher's open access policy
ISSN 0076-6879
Publication date 2012
Sub-type Article (original research)
DOI 10.1016/B978-0-12-396962-0.00003-3
Volume 503
Start page 57
End page 74
Total pages 18
Place of publication Maryland Heights, United States
Publisher Academic Press
Collection year 2013
Language eng
Abstract Peptide-based toxins have attracted much attention in recent years for their exciting potential applications in drug design and development. This interest has arisen because toxins are highly potent and selectively target a range of physiologically important receptors. However, peptides suffer from a number of disadvantages, including poor in vivo stability and poor bioavailability. A number of naturally occurring cyclicpeptides have been discovered in plants, animals, and bacteria that have exceptional stability and potentially ameliorate these disadvantages. The lessons learned from studies of the structures, stabilities, and biological activities of these cyclicpeptides can be applied to the reengineering of toxins that are not naturally cyclic but are amenable to cyclization. In this chapter, we describe solid-phase chemical synthetic methods for the reengineering of peptidetoxins to improve their suitability as therapeutic, diagnostic, or imaging agents. The focus is on small disulfide-rich peptides from the venoms of cone snails and scorpions, but the technology is potentially widely applicable to a number of other peptide-based toxins.
Keyword Chlorotoxin
Peptide synthesis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Issue Title: Protein Engineering for Therapeutics, Part B

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
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Created: Fri, 13 Jan 2012, 15:04:32 EST by Susan Allen on behalf of Institute for Molecular Bioscience