Metagenomic analysis of DNA viruses in a wastewater treatment plant in tropical climate

Tamaki, Hideyuki, Zhang, Rui, Angly, Florent E., Nakamura, Shota, Hong, Pei-Ying, Yasunaga, Teruo, Kamagata, Yoichi and Liu, Wen-Tso (2012) Metagenomic analysis of DNA viruses in a wastewater treatment plant in tropical climate. Environmental Microbiology, 14 2: 441-452. doi:10.1111/j.1462-2920.2011.02630.x


Author Tamaki, Hideyuki
Zhang, Rui
Angly, Florent E.
Nakamura, Shota
Hong, Pei-Ying
Yasunaga, Teruo
Kamagata, Yoichi
Liu, Wen-Tso
Title Metagenomic analysis of DNA viruses in a wastewater treatment plant in tropical climate
Journal name Environmental Microbiology   Check publisher's open access policy
ISSN 1462-2912
1462-2920
Publication date 2012-02
Year available 2011
Sub-type Article (original research)
DOI 10.1111/j.1462-2920.2011.02630.x
Volume 14
Issue 2
Start page 441
End page 452
Total pages 12
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2013
Language eng
Formatted abstract
Viruses have been detected in the different stages of wastewater treatment plants (WWTPs) at concentrations of 108–1010 ml−1 of virus-like particles (VLPs), 10–1000 times higher than in natural aquatic environments, suggesting that WWTPs can be considered as an important reservoir and source of viruses. This study revealed novel diversity and function with the DNA viral communities in the influent, activated sludge, anaerobic digester, and effluent of a domestic WWTP using metagenomics. WWTP was a very specific environment, with less than 5% of the > 936 000 metagenomic sequences obtained (∼70–119 Mbp per sample) similar to sequences present in other environmental viromes. Many viruses found in the WWTP were novel, resulting in only < 5–20% of the reads being phylogenetically or functionally assigned. DNA metabolism was observed as the most abundant function with DNA methylase detected at levels 4.2-fold higher than other published viromes, while carbohydrate and amino acids metabolisms were 3.7- and 4.2-fold less abundant respectively. These specific aspects of the WWTP community functions are likely due to high biomass concentration, turnover rate and microbial activity in WWTPs, and likely include mechanisms that help viruses increase their infectivity. Among ∼500 genotypes estimated in individual WWTP viromes, > 82% were shared. These data suggested that VLPs of most viral types could be present between 1 and 30 days in the process before they were discharged. Viruses in WWTP and the discharged ones can have potential impacts on the functioning of the wastewater treatment system and on the dynamics of microbial community in the surrounding aquatic environments respectively.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 31 October 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
Advanced Water Management Centre Publications
 
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Created: Fri, 13 Jan 2012, 10:08:38 EST by Dr Florent Angly on behalf of School of Chemistry & Molecular Biosciences