Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children

Strachan, Roxanne E., Cornelius, Anita, Gilbert, Gwendolyn L., Gulliver, Tanya, Martin, Andrew, McDonald, Tim, Nixon, Gillian, Roseby, Rob, Ranganathan, Sarath, Selvadurai, Hiran, Smith, Greg, Soto-Martinez, Manuel, Suresh, Sadasivam, Teoh, Laurel, Thapa, Kiran and Wainwright, Claire E. (2012) Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children. Respirology, 17 1: 114-119. doi:10.1111/j.1440-1843.2011.02035.x

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Author Strachan, Roxanne E.
Cornelius, Anita
Gilbert, Gwendolyn L.
Gulliver, Tanya
Martin, Andrew
McDonald, Tim
Nixon, Gillian
Roseby, Rob
Ranganathan, Sarath
Selvadurai, Hiran
Smith, Greg
Soto-Martinez, Manuel
Suresh, Sadasivam
Teoh, Laurel
Thapa, Kiran
Wainwright, Claire E.
Title Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children
Journal name Respirology   Check publisher's open access policy
ISSN 1323-7799
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1111/j.1440-1843.2011.02035.x
Volume 17
Issue 1
Start page 114
End page 119
Total pages 6
Place of publication Richmond, VIC, Australia
Publisher Wiley-Blackwell Publishing Asia
Collection year 2012
Language eng
Formatted abstract
Background and objective:
National surveillance of invasive pneumococcal disease (IPD) includes serotyping Streptococcus pneumoniae (SP) isolates from sterile site cultures. PCR is more sensitive and can identify more SP serotypes (STs) in culture-negative samples. The aim of this study was to determine whether enhanced surveillance of childhood empyema, using PCR, provides additional serotype information compared with conventional surveillance.

Pleural fluid (PF) from children with empyema were cultured and tested by PCR to identify SP, targeting the autolysin gene (lytA). Multiplex PCR-based reverse line blot assay was used to identify SP STs. Corresponding IPD surveillance and serotype data were obtained from the National Notifiable Diseases Surveillance System (NNDSS).


Eighty-nine children with empyema, aged ≤16 years, were recruited between April 2008 and March 2009, inclusive. SP was isolated from 5/84 (5.9%) PF cultures and by PCR in 43/79 (54.4%) PF samples.
Keyword Empyema
Pleural disease
Pneumococcal surveillance
Respiratory infections
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 21 DEC 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Faculty of Health and Behavioural Sciences -- Publications
Official 2012 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
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Created: Fri, 13 Jan 2012, 08:44:10 EST by Matthew Lamb on behalf of School of Medicine