Lamellar leukocyte infiltration and involvement of IL-6 during oligofructose-induced equine laminitis development

Visser, Michelle B. and Pollitt, Christopher C. (2011) Lamellar leukocyte infiltration and involvement of IL-6 during oligofructose-induced equine laminitis development. Veterinary Immunology and Immunopathology, 144 1-2: 120-128. doi:10.1016/j.vetimm.2011.07.016


Author Visser, Michelle B.
Pollitt, Christopher C.
Title Lamellar leukocyte infiltration and involvement of IL-6 during oligofructose-induced equine laminitis development
Journal name Veterinary Immunology and Immunopathology   Check publisher's open access policy
ISSN 0165-2427
1873-2534
Publication date 2011-11-15
Sub-type Article (original research)
DOI 10.1016/j.vetimm.2011.07.016
Volume 144
Issue 1-2
Start page 120
End page 128
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Abstract Laminitis is known to involve deregulation of proteases and destruction of the lamellar basement membrane with the host inflammatory response also playing a role. Leukocyte infiltration has been well characterized in the black walnut model of laminitis induction, but not in carbohydrate induced models. Increased gene expression of multiple cytokines, including IL-6, has also been implicated in laminitis development. Using real time PCR, immunohistochemistry and zymography methods, we characterize leukocyte infiltration and IL-6 gene expression in oligofructose (OF) induced laminitis. As well, we use two in vitro models to investigate a role for IL-6 in protease regulation. Laminitis was induced in normal standardbred horses (n= 5) by alimentary OF dosing and lamellar biopsies were obtained throughout the 48. h experimental period. Lamellar explants and keratinocytes were also isolated from clinically normal horses for in vitro experiments. We found infiltration of calprotectin-positive leukocytes (monocytes and neutrophils) at 18-24. h post oligofructose dosing, while IL-6 gene expression was increased as early as 12. h post dosing. Additionally, while we found that IL-6 did not cause significant BM damage in vitro, it did result in increased secreted proMMP-9 levels from lamellar explants. Thus, we find that leukocyte infiltration does occur during oligofructose-induced laminitis development, however, IL-6 gene expression in the lamellae may precede leukocyte infiltration. Additionally, we show IL-6 plays a role in increasing the level of proMMP-9 in vivo in a manner that does not involve keratinocytes.
Keyword Inflammation
Laminitis
Leukocyte
Il-6
Cytokine
Protease
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 26 July 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 01 Jan 2012, 13:46:07 EST by System User on behalf of School of Veterinary Science