Red and green algal origin of diatom membrane transporters: Insights into environmental adaptation and cell evolution

Chan, Cheong Xin, Reyes-Prieto, Adrian and Bhattacharya, Debashish (2011) Red and green algal origin of diatom membrane transporters: Insights into environmental adaptation and cell evolution. PLoS One, 6 12: e29138.1-e29138.11. doi:10.1371/journal.pone.0029138

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Author Chan, Cheong Xin
Reyes-Prieto, Adrian
Bhattacharya, Debashish
Title Red and green algal origin of diatom membrane transporters: Insights into environmental adaptation and cell evolution
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-12-14
Sub-type Article (original research)
DOI 10.1371/journal.pone.0029138
Open Access Status DOI
Volume 6
Issue 12
Start page e29138.1
End page e29138.11
Total pages 11
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2012
Language eng
Formatted abstract
Membrane transporters (MTs) facilitate the movement of molecules between cellular compartments. The evolutionary
history of these key components of eukaryote genomes remains unclear. Many photosynthetic microbial eukaryotes (e.g.,
diatoms, haptophytes, and dinoflagellates) appear to have undergone serial endosymbiosis and thereby recruited foreign
genes through endosymbiotic/horizontal gene transfer (E/HGT). Here we used the diatoms Thalassiosira pseudonana and
Phaeodactylum tricornutum as models to examine the evolutionary origin of MTs in this important group of marine primary
producers. Using phylogenomics, we used 1,014 diatom MTs as query against a broadly sampled protein sequence database
that includes novel genome data from the mesophilic red algae Porphyridium cruentum and Calliarthron tuberculosum, and
the stramenopile Ectocarpus siliculosus. Our conservative approach resulted in 879 maximum likelihood trees of which 399
genes show a non-lineal history between diatoms and other eukaryotes and prokaryotes (at the bootstrap value $70%). Of
the eukaryote-derived MTs, 172 (ca. 25% of 697 examined phylogenies) have members of both red/green algae as sister
groups, with 103 putatively arising from green algae, 19 from red algae, and 50 have an unresolved affiliation to red and/or
green algae. We used topology tests to analyze the most convincing cases of non-lineal gene history in which red and/or
green algae were nested within stramenopiles. This analysis showed that ca. 6% of all trees (our most conservative estimate)
support an algal origin of MTs in stramenopiles with the majority derived from green algae. Our findings demonstrate the
complex evolutionary history of photosynthetic eukaryotes and indicate a reticulate origin of MT genes in diatoms. We
postulate that the algal-derived MTs acquired via E/HGT provided diatoms and other related microbial eukaryotes the ability
to persist under conditions of fluctuating ocean chemistry, likely contributing to their great success in marine environments.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Full text attached: Copyright: © 2011 Chan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
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Created: Wed, 21 Dec 2011, 12:23:56 EST by Cheong Xin Chan on behalf of Institute for Molecular Bioscience