Quantitative proteomic analysis of the adipocyte plasma membrane

Prior, Matthew J., Larance, Mark, Lawrence, Robert T., Soul, Jamie, Humphrey, Sean, Burchfield, James, Kistler, Carol, Davey, Jonathon R., La-Borde, Penelope J., Buckley, Michael, Kanazawa, Hiroshi, Parton, Robert G., Guihaus, Michael and James, David E. (2011) Quantitative proteomic analysis of the adipocyte plasma membrane. Journal of Proteome Research, 10 11: 4970-4982. doi:10.1021/pr200446r


Author Prior, Matthew J.
Larance, Mark
Lawrence, Robert T.
Soul, Jamie
Humphrey, Sean
Burchfield, James
Kistler, Carol
Davey, Jonathon R.
La-Borde, Penelope J.
Buckley, Michael
Kanazawa, Hiroshi
Parton, Robert G.
Guihaus, Michael
James, David E.
Title Quantitative proteomic analysis of the adipocyte plasma membrane
Journal name Journal of Proteome Research   Check publisher's open access policy
ISSN 1535-3893
1535-3907
Publication date 2011-11-04
Sub-type Article (original research)
DOI 10.1021/pr200446r
Volume 10
Issue 11
Start page 4970
End page 4982
Total pages 13
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2012
Language eng
Abstract The adipocyte is a key regulator of mammalian metabolism. To advance our understanding of this important cell, we have used quantitative proteomics to define the protein composition of the adipocyte plasma membrane (PM) in the presence and absence of insulin. Using this approach, we have identified a high confidence list of 486 PM proteins, 52 of which potentially represent novel cell surface proteins, including a member of the adiponectin receptor family and an unusually high number of hydrolases with no known function. Several novel insulin-responsive proteins including the sodium/hydrogen exchanger, NHE6 and the collagens III and VI were also identified, and we provide evidence of PM-ER association suggestive of a unique functional association between these two organelles in the adipocyte. Together these studies provide a wealth of potential therapeutic targets for the manipulation of adipocyte function and a valuable resource for metabolic research and PM biology.
Keyword Proteomics
GLUT4
Insulin action
NHE6
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
 
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