Liquid serial dilution is inferior to solid media for isolation of cultures representative of the phylum-level diversity of soil bacteria

Schoenborn, Liesbeth, Yates, Penelope S., Grinton, Bronwyn E., Hugenholtz, Philip and Janssen, Peter H. (2004) Liquid serial dilution is inferior to solid media for isolation of cultures representative of the phylum-level diversity of soil bacteria. Applied and Environmental Microbiology, 70 7: 4363-4366. doi:10.1128/AEM.70.7.4363-4366.2004

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Author Schoenborn, Liesbeth
Yates, Penelope S.
Grinton, Bronwyn E.
Hugenholtz, Philip
Janssen, Peter H.
Title Liquid serial dilution is inferior to solid media for isolation of cultures representative of the phylum-level diversity of soil bacteria
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2004-07
Sub-type Article (original research)
DOI 10.1128/AEM.70.7.4363-4366.2004
Open Access Status File (Publisher version)
Volume 70
Issue 7
Start page 4363
End page 4366
Total pages 4
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified versions of the same media. We conclude that, contrary to expectation, liquid serial dilution culture is inferior to culturing on solid media for isolating representatives of many bacterial phyla from soil.
Keyword Bacterial phyla
Pasture soil
Liquid serial dilution
Acidobacteria
Verrucomicrobia
Gemmatimonadetes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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