Sonophoresis-mediated permeation and retention of peptide dendrimers across human epidermis

Mutalik, Srinivas, Nayak, Usha Y., Kalra, Rohit, Kumar, Aravind, Kulkarni, Raghavendra V. and Parekh, Harendra (2012) Sonophoresis-mediated permeation and retention of peptide dendrimers across human epidermis. Skin Research and Technology, 18 1: 101-107. doi:10.1111/j.1600-0846.2011.00539.x

Author Mutalik, Srinivas
Nayak, Usha Y.
Kalra, Rohit
Kumar, Aravind
Kulkarni, Raghavendra V.
Parekh, Harendra
Title Sonophoresis-mediated permeation and retention of peptide dendrimers across human epidermis
Journal name Skin Research and Technology   Check publisher's open access policy
ISSN 0909-752X
Publication date 2012-02
Year available 2011
Sub-type Article (original research)
DOI 10.1111/j.1600-0846.2011.00539.x
Volume 18
Issue 1
Start page 101
End page 107
Total pages 7
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Collection year 2012
Language eng
Formatted abstract
Purpose: The objective of the present study was to assess the effect of sonophoresis on the permeation rate of peptide dendrimers through human skin.

Methods: Peptide dendrimers containing arginine and histidine as terminal amino acids and having varying positive charges (arginine group: R4, R8 and R16 dendrimers, having 4+, 8+ and 16+ charges, respectively; histidinegroup: H4, H8 and H16 dendrimers, having 4+, 8+ and 16+ charges, respectively) were synthesized by Fmoc solidphase peptide synthesis. The in vitro skin permeation studies were conducted using vertical-type diffusion cells and ultrasound was applied using a probe sonicator to the donor solution. The effects of varying concentrations of dendrimer with differing pH of the donor solution on the permeation rate were studied.

Results: All the dendrimers exhibited significantly (P<0.05) higher permeation rates with the application of ultrasound in comparison with passive diffusion studies (without ultrasound). High concentrations of H4 and R4 dendrimers were found in the receptor media compared with other dendrimers at all the concentrations tested, indicating appreciable permeation of low-molecular-weight dendrimers across the skin, assisted by sonophoresis. The opposite was true when assessing dendrimer ‘retention’ in skin, where it was shown to improve upon increasing dendrimer generation/molecular weight. Negligible loss of all the dendrimers (<2%) during skin permeation studies indicates that neither skin nor ultrasound adversely affects the stability of dendrimers.

Conclusion: The present study reveals the successful application of sonophoresis in enhancing the permeation of peptide dendrimers across human skin.
Keyword Dendrimers
Skin Permeation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 20 APR 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Pharmacy Publications
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 29 Nov 2011, 11:16:35 EST by Charna Kovacevic on behalf of School of Pharmacy