Up-regulation of microRNAs in brain of human alcoholics

Lewohl, Joanne M., Nunez, Yury O., Dodd, Peter R., Tiwari, Gayatri R., Harris, R. Adron and Mayfield, R. Dayne (2011) Up-regulation of microRNAs in brain of human alcoholics. Alcoholism: Clinical and Experimental Research, 35 11: 1928-1937. doi:10.1111/j.1530-0277.2011.01544.x


Author Lewohl, Joanne M.
Nunez, Yury O.
Dodd, Peter R.
Tiwari, Gayatri R.
Harris, R. Adron
Mayfield, R. Dayne
Title Up-regulation of microRNAs in brain of human alcoholics
Journal name Alcoholism: Clinical and Experimental Research   Check publisher's open access policy
ISSN 0145-6008
1530-0277
Publication date 2011-11
Sub-type Article (original research)
DOI 10.1111/j.1530-0277.2011.01544.x
Volume 35
Issue 11
Start page 1928
End page 1937
Total pages 10
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
Background: MicroRNAs (miRNAs) are small, noncoding oligonucleotides with an important role in posttranscriptional regulation of gene expression at the level of translation and mRNA degradation. Recent studies have revealed that miRNAs play important roles in a variety of biological processes, such as cell proliferation, neuronal differentiation, developmental timing, synapse function, and neurogenesis. A single miRNA can target hundreds of mRNA transcripts for either translation repression or degradation, but the function of many human miRNAs is not known.
Methods: miRNA array analysis was performed on the prefrontal cortex of 27 individual human cases (14 alcoholics and 13 matched controls). Target genes for differentially expressed miRNAs were predicted using multiple target prediction algorithms and a consensus approach, and predicted targets were matched against differentially expressed mRNAs from the same samples. Over- and under-representation analysis was performed using hypergeometric probability and z-score tests.
Results: Approximately 35 miRNAs were significantly up-regulated in the alcoholic group compared with controls. Target prediction showed a large degree of overlap with our published cDNA microarray data. Functional classification of the predicted target genes of the regulated miRNAs includes apoptosis, cell cycle, cell adhesion, nervous system development, and cell–cell signaling.
Conclusions: These data suggest that the reduced expression of genes in human alcoholic cases may be because of the up-regulated miRNAs. Cellular processes fundamental to neuronal plasticity appear to represent major targets of the suggested miRNA regulation.
Keyword Alcohol
Brain
Gene Expression
MicroRNAs
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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