Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera : Muscidae)

Dudaniec, Rachael Y., Gardner, Michael G. and Kleindorfer, Sonia (2008) Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera : Muscidae). Molecular Ecology Resources, 8 1: 142-144. doi:10.1111/j.1471-8286.2007.01900.x


Author Dudaniec, Rachael Y.
Gardner, Michael G.
Kleindorfer, Sonia
Title Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera : Muscidae)
Journal name Molecular Ecology Resources   Check publisher's open access policy
ISSN 1755-098X
1755-0998
Publication date 2008-01-01
Sub-type Article (original research)
DOI 10.1111/j.1471-8286.2007.01900.x
Volume 8
Issue 1
Start page 142
End page 144
Total pages 3
Place of publication Oxford, England, U. K.
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.
Keyword Darwin's finches
Ectoparasitism
Microsatellites
Multiplex Pcr
Paternity
Philornis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Geography, Planning and Environmental Management Publications
ERA 2012 Admin Only
 
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