A review of beta 2-glycoprotein-I antibody testing results from a peer-driven multilaboratory quality assurance program

Favaloro, Emmanuel J., Wong, Richard C. W., Jovanovich, Susan and Roberts-Thomson, Peter (2007) A review of beta 2-glycoprotein-I antibody testing results from a peer-driven multilaboratory quality assurance program. American Journal of Clinical Pathology, 127 3: 441-448. doi:10.1309/X712-UWH3-7P30-3N62


Author Favaloro, Emmanuel J.
Wong, Richard C. W.
Jovanovich, Susan
Roberts-Thomson, Peter
Title A review of beta 2-glycoprotein-I antibody testing results from a peer-driven multilaboratory quality assurance program
Formatted title
A review of β2-glycoprotein-i antibody testing results from a peer-driven murtilaboratory duality assurance program
Journal name American Journal of Clinical Pathology   Check publisher's open access policy
ISSN 0002-9173
1943-7722
Publication date 2007-03
Sub-type Article (original research)
DOI 10.1309/X712-UWH3-7P30-3N62
Volume 127
Issue 3
Start page 441
End page 448
Total pages 8
Place of publication Birmingham, AL, United States
Publisher American Society for Clinical Pathology
Language eng
Formatted abstract
We evaluated the results of IgG β2-glycoprotein-I (B2GPI) antibody assays in a multilaboratory setting by analyzing data from an external quality assurance program for the 2003 through 2005 cycles for 27 serum samples, including quantitative IgG-B2GPI values and qualitative interpretation and grading (ie, negative or positive; grade of positivity), according to method type and in conjunction with clinical data. We report high interlaboratory variation in numeric IgG-B2GPI results, comparable to that reported for IgG anticardiolipin antibody (aCL) testing, and some method-based variation. For example, interlaboratory coefficients of variation for IgG-B2GPI were more than 50% in 19 samples (70%). For qualitative reporting, there was generally better consensus than previously reported for semiquantitative IgG-aCL testing; although 100% consensus occurred for only 11 samples (41%), more than 90% of laboratories agreed for 19 samples (70%). In some cases, laboratory findings (negative or positive IgG-B2GPI) did not agree with clinical information. Despite the lack of formal standardization for IgG-B2GPI testing compared with IgG-aCL, there seems to be better cross-laboratory consensus. Improvement in standardization of these assays is still required to improve interlaboratory and intermethod concordance of results and interpretation between laboratories and the clinical usefulness of IgG-B2GPI testing.
Keyword Anticardiolipin antibody
Anti╬▓2-glycoprotein-I antibody
Antiphospholipid antibody
Assays
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
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Created: Tue, 15 Nov 2011, 11:43:04 EST by Miss Kristy Reid on behalf of School of Medicine