Telomere length analysis in crustacean species: Metapenaeus macleayi, Sagmariasus verreauxi, and Jasus edwardsii

Godwin, R. M., Frusher, S., Montgomery, S. S. and Ovenden, J. (2011) Telomere length analysis in crustacean species: Metapenaeus macleayi, Sagmariasus verreauxi, and Jasus edwardsii. ICES Journal of Marine Science, 68 10: 2053-2058. doi:10.1093/icesjms/fsr144


Author Godwin, R. M.
Frusher, S.
Montgomery, S. S.
Ovenden, J.
Title Telomere length analysis in crustacean species: Metapenaeus macleayi, Sagmariasus verreauxi, and Jasus edwardsii
Formatted title
Telomere length analysis in crustacean species: Metapenaeus macleayi, Sagmariasus verreauxi, and Jasus edwardsii
Journal name ICES Journal of Marine Science   Check publisher's open access policy
ISSN 1054-3139
1095-9289
Publication date 2011-11
Sub-type Article (original research)
DOI 10.1093/icesjms/fsr144
Volume 68
Issue 10
Start page 2053
End page 2058
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2012
Language eng
Formatted abstract
Estimates of age and growth in crustaceans have been historically problematic and presented significant challenges to researchers. Current techniques of age determination provide valuable data, but also suffer from disadvantages. Telomeric DNA has been proposed as an age biomarker because it shortens with age in some species. In this study, the feasibility of using telomere length (TL) to estimate
age was examined in the school prawn Metapenaeus macleayi and the spiny lobsters Sagmariasus verreauxi and Jasus edwardsii. Carapace length (CL) was used as a surrogate for age, and terminal restriction fragment assays were used to test the relationship between TL and size. Degradation of telomeric DNA with time during storage significantly influenced TL estimates, particularly for M. macleayi. TLs obtained from species in this study were 10–20 kb. No relationship between CL and TL was detected for any of the test species, and TL did not differ between male and female M. macleayi. TLs of J. edwardsii pueruli were unexpectedly shorter than those of J. edwardsii adults. The suitability of TL as an age biomarker in crustaceans may be limited, but further research is needed to elucidate telomere dynamics in these species with their different life histories and lifespans.
Keyword Age biomarker
Age estimation
Australia
Crustacean
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biological Sciences Publications
 
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