High prevalence of human papillomaviruses in fresh frozen breast cancer samples

Antonsson, Annika, Spurr, Terrence P., Chen, Alice C., Francis, Glenn D., McMillan, Nigel A. J., Saunders, Nicholas A., Law, Michael and Bennett, Ian C. (2011) High prevalence of human papillomaviruses in fresh frozen breast cancer samples. Journal of Medical Virology, 83 12: 2157-2163. doi:10.1002/jmv.22223

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Author Antonsson, Annika
Spurr, Terrence P.
Chen, Alice C.
Francis, Glenn D.
McMillan, Nigel A. J.
Saunders, Nicholas A.
Law, Michael
Bennett, Ian C.
Title High prevalence of human papillomaviruses in fresh frozen breast cancer samples
Journal name Journal of Medical Virology   Check publisher's open access policy
ISSN 0146-6615
Publication date 2011-12
Sub-type Article (original research)
DOI 10.1002/jmv.22223
Open Access Status
Volume 83
Issue 12
Start page 2157
End page 2163
Total pages 7
Place of publication Hoboken, NJ, U.S.A.
Publisher John Wiley & Sons
Collection year 2012
Language eng
Abstract While the etiology of breast cancer remains enigmatic, some recent reports have examined the role of human papillomavirus (HPV) in breast carcinogenesis. The purpose of this study was to determine the prevalence of HPV in breast cancer tissue using PCR analysis and sequencing. Fifty-four (54) fresh frozen breast cancers samples that were removed from a cohort of breast cancer patients were analyzed. Samples were tested for HPV using comprehensive PCR primers, and in situ hybridization was performed on paraffin embedded tissue sections. Findings were correlated with clinical and pathological characteristics. The HPV DNA prevalence in the breast cancer samples was 50% (27/54) with sequence analysis indicating all cases to be positive for HPV-18 type. While HPV patients were slightly younger, no correlation was noted for menopausal status or family history. HPV positive tumors were smaller with earlier T staging and demonstrated lesser nodal involvement compared to HPV negative cancers. In situ hybridization analyses proved negative. The high proportion of HPV positive breast cancers detected in this series using fresh frozen tissues cannot be dismissed, however the role of HPV in breast carcinogenesis remains unclear and may ultimately be ascertained by monitoring future breast cancer incidence amongst women vaccinated against high risk HPV types.
Keyword Breast cancer
Human papillomavirus (HPV)
Polymerase chain reaction (PCR)
In situ hybridization
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Medicine Publications
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 22 times in Scopus Article | Citations
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