A pH-responsive coiled-coil peptide hydrogel

Fletcher, Nicholas L., Lockett, Christina V. and Dexter, Annette F. (2011) A pH-responsive coiled-coil peptide hydrogel. Soft Matter, 7 21: 10210-10218. doi:10.1039/c1sm06261a

Author Fletcher, Nicholas L.
Lockett, Christina V.
Dexter, Annette F.
Title A pH-responsive coiled-coil peptide hydrogel
Journal name Soft Matter   Check publisher's open access policy
ISSN 1744-683X
Publication date 2011-09
Sub-type Article (original research)
DOI 10.1039/c1sm06261a
Open Access Status Not Open Access
Volume 7
Issue 21
Start page 10210
End page 10218
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2012
Language eng
Abstract We report the design of an α-helical peptide that forms self-healing hydrogels at weight fractions below 0.1%. Gelation of the 21-residue peptide AFD19 is controlled by changes in pH, whereby highly-charged peptide states give low-viscosity solutions, while thermostable gels form at pH values where the peptide charge lies close to +1 or −1. At pH values where the molecular charge approaches zero, the peptide forms insoluble fibrils. Transitions between the liquid, gel and insoluble states of the peptide are reversible upon addition of acid or base. Electron microscopy, dynamic light scattering and electronic circular dichroism studies indicate that gelation occurs when the peptide self-assembles into soluble coiled-coil fibrils that form cross-links below a critical zeta potential. The formation of extended fibrils occurs in the absence of any explicit design to drive helix offsetting.
Keyword Artificial protein hydrogels
De-novo design
Reversible hydrogels
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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