The effects of skin disease on the penetration kinetics of hydrocortisone through canine skin in vitro

Ahlstrom, Liisa A., Cross, Sheree E. and Mills, Paul C. (2011) The effects of skin disease on the penetration kinetics of hydrocortisone through canine skin in vitro. Veterinary Dermatology, 22 6: 482-489. doi:10.1111/j.1365-3164.2011.00977.x


Author Ahlstrom, Liisa A.
Cross, Sheree E.
Mills, Paul C.
Title The effects of skin disease on the penetration kinetics of hydrocortisone through canine skin in vitro
Formatted title
The effects of skin disease on the penetration kinetics of hydrocortisone through canine skin in vitro
Journal name Veterinary Dermatology   Check publisher's open access policy
ISSN 0959-4493
1365-3164
Publication date 2011-12
Sub-type Article (original research)
DOI 10.1111/j.1365-3164.2011.00977.x
Volume 22
Issue 6
Start page 482
End page 489
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
This study investigated the effects of allergic skin disease on the penetration kinetics of hydrocortisone through canine skin in vitro. Full-thickness lesional and nonlesional (normal) skin was removed from the dorsal lumbosacral and dorsocaudal thoracic regions, respectively, of five canine cadavers. The dogs were suspected of having flea allergy dermatitis based on their distribution and types of skin lesions. Nonlesional skin was confirmed to be histologically normal, and the histopathology of the lesional skin was consistent with allergic dermatitis. Excised skin was clipped, mounted in Franz-type diffusion cells, and the transdermal penetration of a saturated, radiolabelled hydrocortisone solution was measured over 30 h. When the penetration data for all five dogs were pooled, a restricted (or residual) maximal likelihood mixed model predicted that the permeability coefficient and pseudosteady-state flux of hydrocortisone was more than twice as great (95% confidence interval 1.55–2.71 times as great; P < 0.0001) through lesional compared with nonlesional skin. There was no significant difference in the lag time for hydrocortisone penetration through lesional compared with nonlesional skin of the dogs. This study has confirmed that the transdermal penetration of hydrocortisone may be altered, typically increased twofold, but could be as high as 10-fold, through lesional compared with nonlesional skin of dogs with suspected flea allergy dermatitis. This is likely to be affected by variables such as disease severity, concurrent infections and interindividual differences in skin characteristics.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Medicine Publications
School of Veterinary Science Publications
 
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