Elevated iron absorption in the neonatal rat reflects high expression of iron transport genes in the distal alimentary tract

Frazer, David M., Wilkins, Sarah J. and Anderson, Gregory J. (2007) Elevated iron absorption in the neonatal rat reflects high expression of iron transport genes in the distal alimentary tract. American Journal of Physiology: Gastrointestinal and Liver Physiology, 293 3: G525-G531. doi:10.1152/ajpgi.00579.2006


Author Frazer, David M.
Wilkins, Sarah J.
Anderson, Gregory J.
Title Elevated iron absorption in the neonatal rat reflects high expression of iron transport genes in the distal alimentary tract
Journal name American Journal of Physiology: Gastrointestinal and Liver Physiology   Check publisher's open access policy
ISSN 0193-1857
1522-1547
Publication date 2007-09
Sub-type Article (original research)
DOI 10.1152/ajpgi.00579.2006
Volume 293
Issue 3
Start page G525
End page G531
Total pages 7
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Language eng
Formatted abstract
Intestinal iron absorption is extremely high in neonatal mammals but falls rapidly to adult levels following weaning. The aim of this study was to investigate the molecular basis of this elevated neonatal absorption using the rat as an experimental model. RNA was extracted from various sections of the intestine of 10-, 15-, 20-, 25-, and 300-day-old rats and the expression of the genes encoding DMT1 (Slc11a2), ferroportin (Slc40a1), Cybrd1 (Cybrd1), and hephaestin (heph) determined by ribonuclease protection assay. The hepatic expression of Hamp was studied at the same ages. Iron absorption was examined by following 59Fe uptake in both whole animals and in isolated intestinal loops. Slc11a2, Slc40a1, and Cybrd1 mRNAs were highly expressed in all regions of the small intestine and colon studied in suckling rats. However, after weaning, when iron absorption declined significantly, strong expression was retained only in the duodenum. No change in hephaestin mRNA occurred in any part of the digestive tract. In the distal small intestine and colon, Slc40a1 expression most closely followed the change in absorption that occurred after weaning. Hamp expression was low during the neonatal period and increased to adult levels following weaning. Our results suggest that the distal small intestine and colon contribute significantly to the high intestinal iron absorption seen in neonatal animals and that this reflects increased expression of the iron transporters, particularly Slc40a1.
Keyword Slc11a2
Slc40a1
Cybrd1
Hephaestin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
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