Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick

Njiru, Zablon Kithinji (2011) Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick. Diagnostic Microbiology and Infectious Disease, 69 2: 205-209. doi:10.1016/j.diagmicrobio.2010.08.026


Author Njiru, Zablon Kithinji
Title Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick
Journal name Diagnostic Microbiology and Infectious Disease   Check publisher's open access policy
ISSN 0732-8893
1879-0070
Publication date 2011-02
Sub-type Article (original research)
DOI 10.1016/j.diagmicrobio.2010.08.026
Volume 69
Issue 2
Start page 205
End page 209
Total pages 5
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2012
Language eng
Abstract A combined loop-mediated isothermal amplification lateral flow dipstick (LAMP-LFD) format was evaluated in the detection of human infective trypanosome DNA from clinical samples. The LAMP-LFD showed analytical sensitivity equivalent to 0.01 tryps/mL, levels that were identical to using gel electrophoresis and SYBR® Green I dye. The LAMP-LFD showed superior specificity to SYBR® Green I when supernatant prepared from boiled human biological samples was used as template. These results indicate that the use of nonspecific DNA intercalators may produce false positives when partially processed templates are used. The LAMP-LFD format presented here is simple, rapid, and has future potential use in diagnosis of sleeping sickness.
Keyword Loop-mediated isothermal amplification
Lateral flow dipstick
Sleeping sickness
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under Notes

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Veterinary Science Publications
 
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Created: Tue, 08 Nov 2011, 11:12:04 EST by Dr Zablon Njiru on behalf of School of Veterinary Science