Kunjin virus replicon-based vaccines expressing Ebola virus glycoprotein GP protect the guinea pig against lethal Ebola virus infection

Reynard, O., Mokhonov, V., Mokhonova, E., Leung, J., Page, A., Mateo, M., Pyankova, O., Georges-Courbot, M. C., Raoul, H., Khromykh, A. A. and Volchkov, V. E. (2011) Kunjin virus replicon-based vaccines expressing Ebola virus glycoprotein GP protect the guinea pig against lethal Ebola virus infection. Journal of Infectious Diseases, 204 3: S1060-S1065. doi:10.1093/infdis/jir347


Author Reynard, O.
Mokhonov, V.
Mokhonova, E.
Leung, J.
Page, A.
Mateo, M.
Pyankova, O.
Georges-Courbot, M. C.
Raoul, H.
Khromykh, A. A.
Volchkov, V. E.
Title Kunjin virus replicon-based vaccines expressing Ebola virus glycoprotein GP protect the guinea pig against lethal Ebola virus infection
Journal name Journal of Infectious Diseases   Check publisher's open access policy
ISSN 0022-1899
1537-6613
Publication date 2011-11
Sub-type Article (original research)
DOI 10.1093/infdis/jir347
Volume 204
Issue 3
Start page S1060
End page S1065
Total pages 6
Place of publication Cary, NC, United States
Publisher Oxford University Press
Collection year 2012
Language eng
Abstract Pre- or postexposure treatments against the filoviral hemorrhagic fevers are currently not available for human use. We evaluated, in a guinea pig model, the immunogenic potential of Kunjin virus (KUN)–derived replicons as a vaccine candidate against Ebola virus (EBOV). Virus like particles (VLPs) containing KUN replicons expressing EBOV wild-type glycoprotein GP, membrane anchor-truncated GP (GP/Ctr), and mutated GP (D637L) with enhanced shedding capacity were generated and assayed for their protective efficacy. Immunization with KUN VLPs expressing full-length wild-type and D637L-mutated GPs but not membrane anchor–truncated GP induced dose-dependent protection against a challenge of a lethal dose of recombinant guinea pig-adapted EBOV. The surviving animals showed complete clearance of the virus. Our results demonstrate the potential for KUN replicon vectors as vaccine candidates against EBOV infection.
Keyword Marburg virus
Rna
Particles
Primates
System
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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