Recent advances in flow cytometric cell sorting

Osborne, Geoffrey W. (2011). Recent advances in flow cytometric cell sorting. In Zbigniew Darzynkiewicz, Elena Holden, Alberto Orfao, William Telford and Donald Wlodkowic (Ed.), Recent advances in cytometry. Part A: Instrumentation, methods 5th ed. (pp. 533-556) Maryland Heights, MO, United States: Academic Press. doi:10.1016/b978-0-12-374912-3.00021-3

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Author Osborne, Geoffrey W.
Title of chapter Recent advances in flow cytometric cell sorting
Title of book Recent advances in cytometry. Part A: Instrumentation, methods
Place of Publication Maryland Heights, MO, United States
Publisher Academic Press
Publication Year 2011
Sub-type Chapter in textbook
DOI 10.1016/b978-0-12-374912-3.00021-3
Open Access Status
Series Methods in Cell Biology
Edition 5th
ISBN 9780123749123
ISSN 0091-6579
Editor Zbigniew Darzynkiewicz
Elena Holden
Alberto Orfao
William Telford
Donald Wlodkowic
Volume number 102
Chapter number 21
Start page 533
End page 556
Total pages 600
Total chapters 21
Collection year 2012
Language eng
Formatted Abstract/Summary
The classification and separation of one cell type or particle from others is a fundamental task in many areas of science. Numerous techniques are available to perform this task; however, electrostatic cell sorting has gained eminence over others because, when combined with the analysis capabilities of flow cytometry it provides flexible separations based on multiple parameters. Unlike competing technologies, such as gradient or magnetic separations that offer much larger total throughput, flow cytometric cell sorting permits selections based on various levels of fluorescent reporters, rather the complete presence or absence of the reporter. As such, this technology has found application in a huge range of fields. This chapter aims to describe the utility of single-cell sorting with particular emphasis given to index sorting. This is followed by two recently developed novel techniques of sorting cells or particles. The first of these is positional sorting which is useful in cell-based studies where sorting can proceed and produce meaningful results without being inherently dependant on prior knowledge of where gates should be set. Secondly, reflective plate sorting is introduced which positionally links multiwell sample and collection plates in a convenient assay format so that cells in the collection plate “reflect” those in the sample plate.
Keyword Bivariate plot
Cell sorting
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 24 June 2011.

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Created: Fri, 04 Nov 2011, 16:12:25 EST by Debra McMurtrie on behalf of Queensland Brain Institute