Caprine plasma proteinase inhibitors. I. Partial characterization

Vankan, D. M. and Bell, K. (1993) Caprine plasma proteinase inhibitors. I. Partial characterization. Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology, 104 1: 101-108. doi:10.1016/0305-0491(93)90344-5


Author Vankan, D. M.
Bell, K.
Title Caprine plasma proteinase inhibitors. I. Partial characterization
Journal name Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology   Check publisher's open access policy
ISSN 1096-4959
1879-1107
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1016/0305-0491(93)90344-5
Volume 104
Issue 1
Start page 101
End page 108
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
Using two-dimensional electrophoresis (IEF, pH 3.5-0.0 and PAGE, 11.5% T, pH 7.9) the caprine plasma proteinase inhibitors were classified into six distinct classes, designated PIA, PIB, PIC, PID, PIE and PIF. Differentiation of the six inhibitors was based on electrophoretic criteria, their abilities to inhibit bovine trypsin and chymotrypsin and their cross reactions with antisera to human α1-antitrypsin and α1-antichymotryspin. Polymorphic variants were identified for five of the protein systems (PIA, PIB, PIC, PID and PIE) and the electrophoretic data indicated that the variants were controlled by allelic genes. PIF proteins were poorly resolved and invariant. Treatment of selected plasmas with neuraminidase demonstrated that the microheterogeneity observed in the PIA, PIB, PIC and PID proteins was attributable to sialic acid additions. The inhibitory activities of all six caprine proteinase inhibitors were unaffected by chemical oxidation with chloramine-T.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Thu, 03 Nov 2011, 02:38:44 EST by Dianne Vankan on behalf of Centre for Companion Animal Health