Single-cell analysis of the RhD blood type for use in preimplantation diagnosis in the prevention of severe hemolytic disease of the newborn

Van den Veyverr, Ignatia B., Chong, Samuel S., Cota, Juan, Bennett, Phillip R., Fisk, Nicholas M., Handyside, Alan H., Cartron, Jean-Pierre, Le Van Kim, Caroline, Colin, Yves, Snabes, Michael C., Moise, Kenneth J. and Hughes, Mark R. (1995) Single-cell analysis of the RhD blood type for use in preimplantation diagnosis in the prevention of severe hemolytic disease of the newborn. American Journal of Obstetrics and Gynecology, 172 2: 533-540. doi:10.1016/0002-9378(95)90569-3


Author Van den Veyverr, Ignatia B.
Chong, Samuel S.
Cota, Juan
Bennett, Phillip R.
Fisk, Nicholas M.
Handyside, Alan H.
Cartron, Jean-Pierre
Le Van Kim, Caroline
Colin, Yves
Snabes, Michael C.
Moise, Kenneth J.
Hughes, Mark R.
Title Single-cell analysis of the RhD blood type for use in preimplantation diagnosis in the prevention of severe hemolytic disease of the newborn
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
ISSN 0002-9378
1097-6868
Publication date 1995-02
Sub-type Article (original research)
DOI 10.1016/0002-9378(95)90569-3
Volume 172
Issue 2
Start page 533
End page 540
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Mosby
Language eng
Formatted abstract
Objective:
Our purpose was to develop a molecular assay to determine the fetal RhD blood type on single diploid cells, including blastomeres.

Study design:

Polymerase chain reaction amplification of a 99 bp deoxyribonucleic acid fragment of the RhD gene or a 113 bp fragment from the RhCE gene was performed from 20 venous blood samples and 20 amniotic fluid samples and from 60 single-cultured lymphoblasts and 12 media blanks mixed in a blinded fashion. This reaction was similarly tested after whole-genome amplification on 10 lymphoblasts and seven human blastomeres.

Results:

Deoxyribonucleic acid amplification was successful and correct from all genomic deoxyribonucleic acid samples. Ninety-seven percent of single cells amplified, correct diagnosis was made in 96%. Five blastomeres successfully amplified. No media blanks produced amplified, contaminating deoxyribonucleic acid.

Conclusions:
The RhD blood type can be determined reliably from single cells and can be used for preimplantation genetic diagnosis for the prevention of rhesus hemolytic disease.
Keyword Preimplantation diagnosis
Rhd blood type
Polymerase chain reaction
Hemolytic disease of the newborn
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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