Absence of expression of RhD by human trophoblast cells

Benachi, Alexandra, Garritsen, Henk S.P., Howard, Catherine M., Bennett, Phillip and Fisk, Nicholas M. (1998) Absence of expression of RhD by human trophoblast cells. American Journal of Obstetrics and Gynecology, 178 2: 294-299. doi:10.1016/S0002-9378(98)80015-X


Author Benachi, Alexandra
Garritsen, Henk S.P.
Howard, Catherine M.
Bennett, Phillip
Fisk, Nicholas M.
Title Absence of expression of RhD by human trophoblast cells
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
ISSN 0002-9378
1097-6868
Publication date 1998-02
Sub-type Article (original research)
DOI 10.1016/S0002-9378(98)80015-X
Volume 178
Issue 2
Start page 294
End page 299
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Mosby
Language eng
Formatted abstract
OBJECTIVE:
Our purpose was to determine whether the RhO gene is expressed in trophoblast at any stage of gestation.

STUDY DESIGN:
Trophoblast and fetal tissue were obtained from 18 pregnancies at 8 to 40 weeks' gestation. Deoxyribonucleic acid and ribonucleic acid were extracted from trophoblast. Complementary deoxyribonucleic acid was synthesized from ribonucleic acid, and reverse transcriptase-polymerase chain reaction was performed using primers specific for the RhD gene. Deoxyribonucleic acid was extracted from fetal tissue to determine the fetal RhD status by means of polymerase chain reaction. Antigen expression was also sought by analytic cytometric analysis (flow cytometry and immunocytochemistry) using a monoclonal anti-D antibody.

RESULTS:
Trophoblast was studied from various combinations of RhD-positive and RhD-negative fetuses (on deoxyribonucleic acid) from mothers to find no RhD gene expression in any sample. Flow cytometry and immunocytochemistry confirmed this by demonstrating no RhD antigen sites on trophoblast cells.

CONCLUSION:
Contrary to a previous report, we conclude that the RhD gene is not expressed in human trophoblast in any trimester.
Keyword Rhesus D expression
Human trophoblast
Blood
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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