Detection of fetal messenger ribonucleic acid in maternal blood to determine fetal RhD status as a strategy for noninvasive prenatal diagnosis

AL-Mufti, Raghad, Howard, Catherine, Overton, Timothy, Holzgreve, Wolfgang, Gaenshirt, Dorothee, Fisk, Nicholas M. and Bennett, Phillip (1998) Detection of fetal messenger ribonucleic acid in maternal blood to determine fetal RhD status as a strategy for noninvasive prenatal diagnosis. American Journal of Obstetrics and Gynecology, 179 1: 210-214. doi:10.1016/S0002-9378(98)70274-1


Author AL-Mufti, Raghad
Howard, Catherine
Overton, Timothy
Holzgreve, Wolfgang
Gaenshirt, Dorothee
Fisk, Nicholas M.
Bennett, Phillip
Title Detection of fetal messenger ribonucleic acid in maternal blood to determine fetal RhD status as a strategy for noninvasive prenatal diagnosis
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
ISSN 0002-9378
1097-6868
Publication date 1998-07
Sub-type Article (original research)
DOI 10.1016/S0002-9378(98)70274-1
Volume 179
Issue 1
Start page 210
End page 214
Total pages 5
Place of publication Philadelphia, PA, United States
Publisher Mosby
Language eng
Formatted abstract
OBJECTIVES:
Our purpose was to test the hypothesis that reverse transcriptase-polymerase chain reaction for fetal messenger ribonucleic acid in maternal blood is more sensitive than polymerase chain reaction from genomic deoxyribonucleic acid in prenatal determination of fetal RhD blood type.

STUDY DESIGN:
Fetal nucleated erythrocytes in peripheral blood from 35 RhD-negative women were enriched by triple-density gradient centrifugation, anti-CD71 magnetic sorted, and deoxyribonucleic acid and ribonucleic acid extracted. Sensitivities of reverse transcriptase-polymerase chain reaction and polymerase chain reaction were compared to predict definitive fetal RhD blood type determined in fetal tissues.

RESULTS:

Reverse transcriptase- polymerase chain reaction was significantly more accurate (P = .03) than genomic-polymerase chain reaction in predicting fetal RhD blood type, both overall (28 of 35 vs 22 of 35) and when the fetus was RhD-positive (12 of 19 vs 6 of 19).

CONCLUSIONS:
Reverse transcriptase-polymerase chain reaction is more sensitive than genomic-polymerase chain reaction in detection of fetal RhD sequences in maternal blood.
Keyword Rh alloimmunization
Rh blood group antigens
Polymerase chain reaction
Prenatal diagnosis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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