Ex vivo delineation of placental angioarchitecture with the microbubble contrast agent Levovist

Denbow, Mark L., Eckersley, Robert, Welsh, Alec W., Taylor, Myles J., Carter, Rick C., Cosgrove, David O. and Fisk, Nicholas M. (2000) Ex vivo delineation of placental angioarchitecture with the microbubble contrast agent Levovist. American Journal of Obstetrics and Gynecology, 182 4: 966-971. doi:10.1016/S0002-9378(00)70355-3

Author Denbow, Mark L.
Eckersley, Robert
Welsh, Alec W.
Taylor, Myles J.
Carter, Rick C.
Cosgrove, David O.
Fisk, Nicholas M.
Title Ex vivo delineation of placental angioarchitecture with the microbubble contrast agent Levovist
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
ISSN 0002-9378
Publication date 2000-04
Sub-type Article (original research)
DOI 10.1016/S0002-9378(00)70355-3
Volume 182
Issue 4
Start page 966
End page 971
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Mosby
Language eng
Formatted abstract
The aim of this study was to delineate placental vasculature with the microbubble contrast agent Levovist (99.9% galactose and 0.1% palmitic acid; Schering AG, Berlin, Germany), with the ultimate goal of delineating placental vascular anatomy in utero.

A placental lobule from each of 11 term human placentas was perfused on the fetal side of the circulation under physiologic conditions. Randomly assigned dose- concentration combinations of Levovist were administered through a chorionic artery into the corresponding placental lobule, and the resultant echoenhancement with power Doppler imaging was recorded for digital analysis. Interplacental variability was corrected for by averaging the results of three injections at each dose-concentration combination.


Echoenhancement was seen at all dose-concentration combinations in the injected lobule but not in adjacent control lobules. The three dose- concentration combinations that achieved optimal maximal integrated intensity and duration of action for both chorionic vessel and villus enhancement were 100 μL/kg of 400-mg/mL Levovist, 200 μL/kg of 400-mg/mL Levovist, and 400 μL/kg of 200-mg/mL Levovist.

Microbubble contrast injection into the fetal vasculature enabled power Doppler imaging echoenhancement both in chorionic vessels and within the villus tree. We speculate that fetal injection of contrast agent may be applied to the delineation of placental lesions or areas of interfetal transfusion, although its applicability will be hindered by the need for fetal blood sampling.
Keyword Digital analysis
Microbubble contrast
Placental vascular anatomy
Twin transfusion syndrome
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
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