Studies on sildenafil citrate (Viagra) interaction with DNA using electrochemical DNA biosensor

Rauf, Sakandar, Nawaz, Haq, Akhtar, Kalsoom, Ghauri, Muhammad A. and Khalid, Ahmad M. (2007) Studies on sildenafil citrate (Viagra) interaction with DNA using electrochemical DNA biosensor. Biosensors and Bioelectronics, 22 11: 2471-2477. doi:10.1016/j.bios.2006.09.023


Author Rauf, Sakandar
Nawaz, Haq
Akhtar, Kalsoom
Ghauri, Muhammad A.
Khalid, Ahmad M.
Title Studies on sildenafil citrate (Viagra) interaction with DNA using electrochemical DNA biosensor
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2007-05
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.bios.2006.09.023
Volume 22
Issue 11
Start page 2471
End page 2477
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
The interaction of sildenafil citrate (Viagra) with DNA was studied by using an electrochemical DNA biosensor. The binding mechanism of sildenafil citrate was elucidated by using constant current potentiometry and differential pulse voltammetry at DNA-modified glassy carbon electrode. The decrease in the guanine oxidation peak area or peak current was used as an indicator for the interaction in 0.2 M acetate buffer (pH 5). The binding constant (K) values obtained were 2.01 ± 0.05 × 105 and 1.97 ± 0.01 × 105 M−1 with constant current potentiometry and differential pulse voltammetry, respectively. A linear dependence of the guanine peak area or peak current was observed within the range of 1–40 μM sildenafil citrate with slope = −2.74 × 10−4 s/μM, r = 0.989 and slope = −2.78 × 10−3 μA/μM, r = 0.995 by using constant current potentiometry and differential pulse voltammetry, respectively. Additionally, binding constant values for sildenafil citrate–DNA interaction were determined for the pH range of 4–8 and in biological fluids (serum and urine) at pH 5. The influence of sodium and calcium ions was also studied to elucidate the mechanism of sildenafil citrate–DNA interaction under different solution conditions. The present study may prove to be helpful in extending our understanding of the anticancer activity of sildenafil citrate from cellular to DNA level.
Keyword Electrochemical DNA biosensor
Sildenafil citrate (Viagra)
Glassy carbon electrode
Constant current potentiometry
Differential pulse voltammetry
UV-Vis spectroscopy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
Australian Institute for Bioengineering and Nanotechnology Publications
 
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