European wild boars and domestic pigs display different polymorphic patterns in the Toll-like receptor (TLR) 1, TLR2, and TLR6 genes

Bergman, Ingrid-Maria, Rosengren, Johan K., Edman, Kjell and Edfors, Inger (2010) European wild boars and domestic pigs display different polymorphic patterns in the Toll-like receptor (TLR) 1, TLR2, and TLR6 genes. Immunogenetics, 62 1: 49-52. doi:10.1007/s00251-009-0409-4


Author Bergman, Ingrid-Maria
Rosengren, Johan K.
Edman, Kjell
Edfors, Inger
Title European wild boars and domestic pigs display different polymorphic patterns in the Toll-like receptor (TLR) 1, TLR2, and TLR6 genes
Journal name Immunogenetics   Check publisher's open access policy
ISSN 0093-7711
1432-1211
Publication date 2010-01
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s00251-009-0409-4
Volume 62
Issue 1
Start page 49
End page 52
Total pages 4
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
During the last decade, the Toll-like receptors (TLRs) have been extensively studied, and their immense importance in innate immunity is now being unveiled. Here, we report pronounced differences—probably reflecting the domestication process and differences in selective pressure—between wild boars and domestic pigs regarding single nucleotide polymorphisms (SNPs) in TLR genes. The open reading frames of TLR1, TLR2, and TLR6 were sequenced in 25 wild boars, representing three populations, and in 15 unrelated domestic pigs of Hampshire, Landrace, and Large White origin. In total, 20, 27, and 26 SNPs were detected in TLR1, TLR2, and TLR6, respectively. In TLR1 and TLR2, the numbers of SNPs detected were significantly lower (P ≤ 0.05, P ≤ 0.01) in the wild boars than in the domestic pigs. In the wild boars, one major high frequency haplotype was found in all three genes, while the same pattern was exhibited only by TLR2 in the domestic pigs. The relative frequency of non-synonymous (dN) and synonymous (dS) SNPs was lower for the wild boars than for the domestic pigs in all three genes. In addition, differences in diversity between the genes were revealed: the mean heterozygosity at the polymorphic positions was markedly lower in TLR2 than in TLR1 and TLR6. Because of its localization—in proximity of the bound ligand—one of the non-synonymous SNPs detected in TLR6 may represent species-specific function on the protein level. Furthermore, the codon usage pattern in the genes studied deviated from the general codon usage pattern in Sus scrofa.
Keyword Polymorphism
Toll-like receptor (TLR)
Selection
Swine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published online: 2 December 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Biomedical Sciences Publications
 
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Created: Sat, 22 Oct 2011, 08:25:25 EST by Dr Johan Rosengren on behalf of School of Biomedical Sciences