Lactococcus lactis catalyses electricity generation at microbial fuel cell anodes via excretion of a soluble quinone

Freguia, Stefano, Masuda, Masaki, Tsujimura, Seiya and Kano, Kenji (2009) Lactococcus lactis catalyses electricity generation at microbial fuel cell anodes via excretion of a soluble quinone. Bioelectrochemistry, 76 1-2: 14-18. doi:10.1016/j.bioelechem.2009.04.001


Author Freguia, Stefano
Masuda, Masaki
Tsujimura, Seiya
Kano, Kenji
Title Lactococcus lactis catalyses electricity generation at microbial fuel cell anodes via excretion of a soluble quinone
Formatted title
Lactococcus lactis catalyses electricity generation at microbial fuel cell anodes via excretion of a soluble quinone
Journal name Bioelectrochemistry   Check publisher's open access policy
ISSN 1567-5394
1878-562X
Publication date 2009-09
Sub-type Article (original research)
DOI 10.1016/j.bioelechem.2009.04.001
Volume 76
Issue 1-2
Start page 14
End page 18
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Lactococcus lactis is a gram-positive, normally homolactic fermenter that is known to produce several kinds of membrane associated quinones, which are able to mediate electron transfer to extracellular electron acceptors such as Fe3+, Cu2+ and hexacyanoferrate. Here we show that this bacterium is also capable of performing extracellular electron transfer to anodes by utilizing at least two soluble redox mediators, as suggested by the two-step catalytic current developed. One of these two mediators was herein suggested to be 2-amino-3-dicarboxy-1,4-naphthoquinone (ACNQ), via evaluation of standard redox potential, ability of the bacterium to exploit the quinone when exogenously provided, as well as by high performance liquid chromatography coupled with UV spectrum analysis. During electricity generation, L. lactis slightly deviated from its normal homolactic metabolism by excreting acetate and pyruvate in stoichiometric amounts with respect to the electrical current. In this metabolism, the anode takes on the role of electron sink for acetogenic fermentation. The finding that L. lactis self-catalyses anodic electron transfer by excretion of redox mediators is remarkable as the mechanisms of extracellular electron transfer by pure cultures of gram-positive bacteria had previously never been elucidated.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
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Created: Sat, 22 Oct 2011, 08:15:34 EST by Stefano Freguia on behalf of School of Chemical Engineering