Campylobacter fetus subsp. venerealis small animal infection model

Koya, Ameera, Boe-Hansen, Gry, deWet, Sharon, Turner, Shirley, Cawdell-Smith, A. Judith, Greer, Ristan, Lew-Tabor, Ala and Venus, Bronwyn (2011). Campylobacter fetus subsp. venerealis small animal infection model. In: ASM 2011 Australian Society for Microbiology (ASM) Annual Scientific Meeting, Hobart, Australia, (). 4-8 July 2011.

Author Koya, Ameera
Boe-Hansen, Gry
deWet, Sharon
Turner, Shirley
Cawdell-Smith, A. Judith
Greer, Ristan
Lew-Tabor, Ala
Venus, Bronwyn
Title of paper Campylobacter fetus subsp. venerealis small animal infection model
Conference name ASM 2011 Australian Society for Microbiology (ASM) Annual Scientific Meeting
Conference location Hobart, Australia
Conference dates 4-8 July 2011
Publication Year 2011
Sub-type Oral presentation
Language eng
Abstract/Summary Bovine genital campylobacteriosis (BGC), caused by Campylobacter fetus subsp. venerealis (Cfv), is associated with production losses in the Australian meat and dairy industry. The objective of this pilot study was to develop a reliable guinea pig model for BGC abortion to facilitate future studies of Cfv pathogenicity, abortion mechanism and vaccine efficacy. Four groups (A-D) of 5 guinea pigs (1 control per group) at 42 days gestation were inoculated with laboratory isolates of Cfv via intra-peritoneal (IP) injection. Groups A, B, C and D were injected with four different isolates; Q41 at 8.4x107 cfu/ml, 258 at 1x108 cfu/ml, 540 at 1x108 cfu/ml, and 540 at 1.2x104 cfu/ml respectively. Tissues from dams and aborted foetuses were examined using culture and histology. The study was approved by the University of Queensland Animal Ethics Committee. Groups B and C had abortion rates of 100% and 75% respectively within a time period of 19-168 hours while Group A produced no abortions within 7 days of IP injection. The occurrence of Cfv isolated from peritoneum, uterus horns, placental sites, amniotic fluid and foetal stomach content varied. One dam in Group D aborted one foetus. No control animals aborted and Cfv was not isolated in Group D and the controls. Histologicial examination showed tissue changes associated with placentitis, suggesting that Cfv induces inflammation, placental detachment and abortion. Strain virulence variation appears to be present. Intra-peritoneal administration of Cfv to pregnant guinea pigs is a promising small animal model for investigation of BCG abortion.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Conference Paper
Collections: Queensland Alliance for Agriculture and Food Innovation
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Created: Fri, 21 Oct 2011, 23:46:30 EST by Dr Alicja Lew-tabor on behalf of Qld Alliance for Agriculture and Food Innovation