Accumulation of murine subretinal macrophages: effects of age, pigmentation and CX(3)CR1

Chinnery, Holly R., McLenachan, Samuel, Humphries, Timothy, Kezic, Jelena M., Chen, Xiangting, Ruitenberg, Marc J. and McMenamin, Paul G. (2012) Accumulation of murine subretinal macrophages: effects of age, pigmentation and CX(3)CR1. Neurobiology of Aging, 33 8: 1769-1776.


Author Chinnery, Holly R.
McLenachan, Samuel
Humphries, Timothy
Kezic, Jelena M.
Chen, Xiangting
Ruitenberg, Marc J.
McMenamin, Paul G.
Title Accumulation of murine subretinal macrophages: effects of age, pigmentation and CX(3)CR1
Formatted title Accumulation of murine subretinal macrophages: effects of age, pigmentation and CX3CR1
Journal name Neurobiology of Aging  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2012-08
Sub-type Article
Year available 2011
DOI 10.1016/j.neurobiolaging.2011.03.010
Volume number 33
Issue number 8
ISSN 0197-4580
1558-1497
Start page 1769
End page 1776
Total pages 8
Place of publication Philadelphia, PA , United States
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract Macrophages or activated microglia in the subretinal space are considered a hallmark of some retinal pathologies. We investigated the effects of age, pigmentation and CX3CR1 deficiency on the accumulation of macrophages/activated microglia in the outer retina of young and old Cx3 cr1gfp/gfp (CX3CR1-deficient) or Cx3 cr1gfp/+ mice on either a pigmented (C57BL/6) or albino (BALB/c) background. Quantitative analysis of immunostained retinal-choroidal whole mounts revealed an increase in subretinal macrophage (SRMΦ) numbers in young Cx3 cr1gfp/gfp mice compared with Cx3 cr1gfp/+ mice, however the increase was more marked in albino Cx3 cr1gfp/gfp mice. In aged mice, large numbers of SRMΦ/activated microglia replete with autofluorescent debris were noted in both old pigmented Cx3 cr1gfp/gfp and Cx3 cr1gfp/+ mice proving this accumulation was not CX3CR1-dependent. While CX3CR1 deficiency leads to an early onset of SRMΦ accumulation, our data reveal that this change occurs in both aged Cx3 cr1gfp/+ and Cx3 cr1gfp/gfp pigmented mice in the absence of marked retinal degeneration and is likely a normal response to aging.
Keyword Aging
CX3CR1
Macrophages
Microglia
Retina
Retinal degeneration
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 13 May 2011.

Document type: Journal Article
Sub-type: Article
Collections: Official 2012 Collection
School of Biomedical Sciences Publications
 
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Created: Fri, 21 Oct 2011, 17:14:50 EST by Marc Ruitenberg on behalf of School of Biomedical Sciences