A bedside assay to detect streptococcus pneumoniae in children with empyema

Strachan, Roxanne E., Cornelius, Anita, Gilbert, Gwendolyn L., Gulliver, Tanya, Martin, Andrew, McDonald, Tim, Nixon, Gillian, Roseby, Rob, Ranganathan, Sarath, Selvadurai, Hiran, Smith, Greg, Soto-Martinez, Manuel, Suresh, Sadasivam, Teoh, Laurel, Thapa, Kiran, Wainwright, Claire E. and Jaffe, Adam (2011) A bedside assay to detect streptococcus pneumoniae in children with empyema. Pediatric Pulmonology, 46 2: 179-183. doi:10.1002/ppul.21349

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Author Strachan, Roxanne E.
Cornelius, Anita
Gilbert, Gwendolyn L.
Gulliver, Tanya
Martin, Andrew
McDonald, Tim
Nixon, Gillian
Roseby, Rob
Ranganathan, Sarath
Selvadurai, Hiran
Smith, Greg
Soto-Martinez, Manuel
Suresh, Sadasivam
Teoh, Laurel
Thapa, Kiran
Wainwright, Claire E.
Jaffe, Adam
Title A bedside assay to detect streptococcus pneumoniae in children with empyema
Journal name Pediatric Pulmonology   Check publisher's open access policy
ISSN 8755-6863
Publication date 2011-02
Year available 2010
Sub-type Article (original research)
DOI 10.1002/ppul.21349
Volume 46
Issue 2
Start page 179
End page 183
Total pages 5
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2012
Language eng
Formatted abstract
Empyema is a complication of pneumonia, commonly caused by Streptococcus pneumoniae.

To validate the utility of an immunochromatographic test for the detection of S. pneumoniae antigen in the pleural fluid of children with empyema.

Empyema patients had blood and pleural fluid cultured, and polymerase chain reaction (PCR) to detect the S. pneumoniae autolysin gene, lytA, in pleural fluid. Pleural fluid was tested using the Binax NOW S. pneumoniae antigen detection assay and compared with lytA PCR results and/or culture in blood or pleural fluid.

S. pneumoniae was detected by PCR in pleural fluid of 68 of 137 (49.6%) patients, by culture in 11 of 135 (8.1%) pleural specimens and 16 of 120 (13.3%) blood specimens. Pleural fluid Binax NOW testing from 130 patients demonstrated a sensitivity of 83.8% and specificity of 93.5% (positive predictive value of 93.4% and negative predictive value of 84.1%).


In pediatric empyema, high predictive values of pleural fluid Binax NOW S. pneumoniae antigen test suggest that this test may help rationalize antibiotic choice in these patients.
Keyword Pediatric
Pleural empyema
Polymerase chain reaction
Streptococcus pneumoniae
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes On behalf of the Australian Research Network in Empyema (ARNiE)

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Medicine Publications
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Created: Fri, 21 Oct 2011, 15:48:35 EST by Claire Wainwright on behalf of Paediatrics & Child Health - RBWH