Prothrombin G20210A is a bifunctional gene polymorphism

Carter, A.M., Sachchithananthan, M., Stasinopoulos, S., Maurer, F. and Medcalf, R.L. (2002) Prothrombin G20210A is a bifunctional gene polymorphism. Thrombosis and Haemostasis, 87 5: 846-853.

Author Carter, A.M.
Sachchithananthan, M.
Stasinopoulos, S.
Maurer, F.
Medcalf, R.L.
Title Prothrombin G20210A is a bifunctional gene polymorphism
Journal name Thrombosis and Haemostasis   Check publisher's open access policy
ISSN 0340-6245
Publication date 2002
Sub-type Article (original research)
Volume 87
Issue 5
Start page 846
End page 853
Total pages 8
Place of publication Stuttgart, Germany
Publisher Schattauer GmbH
Language eng
Abstract The G20210A polymorphism has been shown to alter the efficiency of prothrombin mRNA processing. Here we show that the G20210A mutation also alters prothrombin mRNA stability. Three-fold more prothrombin protein and mRNA were produced in NIH-3T3 cells transfected with the prothrombin cDNAs containing the 20210A variant compared to cells expressing the 20210G variant. mRNA stability assays using chimeric globin transcripts harboring the G or A variant of the 97 nt prothrombin 3′ UTR indicated that the 20210G variant conferred greater instability to the globin reporter transcript than the A variant in transfected HepG2 cells. Both variants of the prothrombin 3′-UTR were shown to provide binding sites for a number of cellular proteins including HuR, an RNA binding protein associated with mRNA stability. Our results indicate that the G20210A is a bifunctional polymorphism, as it not only alters the efficiency of mRNA processing, but also the decay rate of prothrombin mRNA.
Keyword G20210A
mRNA stability
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Fri, 21 Oct 2011, 14:55:48 EST by Mythily Mariasegaram on behalf of UQ Centre for Clinical Research