Efficient shoot regeneration and Agrobacterium-mediated transformation of Brassica juncea

Zhang, YuXiu, Xu, Jin, Han, Lu, Wei, Wei, Guan, ZiQiu, Cong, Lin and Chai, Tuanyao (2006) Efficient shoot regeneration and Agrobacterium-mediated transformation of Brassica juncea. Plant Molecular Biology Reporter, 24 2: 225a-225i.

Author Zhang, YuXiu
Xu, Jin
Han, Lu
Wei, Wei
Guan, ZiQiu
Cong, Lin
Chai, Tuanyao
Title Efficient shoot regeneration and Agrobacterium-mediated transformation of Brassica juncea
Formatted title
Efficient shoot regeneration and Agrobacterium-mediated transformation of Brassica juncea
Journal name Plant Molecular Biology Reporter   Check publisher's open access policy
ISSN 0735-9640
1572-9818
Publication date 2006-06
Sub-type Article (original research)
Volume 24
Issue 2
Start page 225a
End page 225i
Total pages 9
Place of publication New York, United States
Publisher Springer New York LLC
Language eng
Formatted abstract
Procedures for callus induction, plantlet regeneration, and Agrobacterium-mediated transformation of Brassica juncea were optimized by studying several factors, including explant types, and various plant growth regulators and adjuvants, such as silver nitrate, sucrose and agar. The highest shoot regeneration frequency was obtained from hypocotyl and cotyledonary petiole explants on MS medium containing 3 mg/L benzylaminopurine (BA) and 2 mg/L α-naphthaleneacetic acid (NAA). Transformation was affected by a number of factors, including explant type, selection agents, preculture duration, pre-selection conditions, and coculture temperature. Transformation efficiencies for hypocotyl and cotyledonary petiole explants were at 65% and 69%, respectively.
Keyword Indian mustard
In vitro culture
Explant
Plant growth regulator
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published under Publish by Abstract. This article, in detail, is available only in the electronic version of the Plant Molecular Biology Reporter.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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Created: Fri, 21 Oct 2011, 12:36:31 EST by Wei Wei on behalf of Queensland Brain Institute