Gene manipulation of a heavy metal hyperaccumulator species Thlaspi caerulescens L. via Agrobacterium-mediated transformation

Guan, Zi Qiu, Chai, Tuan Yao, Zhang, Yu Xiu, Xu, Jin, Wei, Wei, Han, Lu and Cong, Lin (2008) Gene manipulation of a heavy metal hyperaccumulator species Thlaspi caerulescens L. via Agrobacterium-mediated transformation. Molecular Biotechnology, 40 1: 77-86. doi:10.1007/s12033-008-9065-4


Author Guan, Zi Qiu
Chai, Tuan Yao
Zhang, Yu Xiu
Xu, Jin
Wei, Wei
Han, Lu
Cong, Lin
Title Gene manipulation of a heavy metal hyperaccumulator species Thlaspi caerulescens L. via Agrobacterium-mediated transformation
Formatted title
Gene manipulation of a heavy metal hyperaccumulator species Thlaspi caerulescens L. via Agrobacterium-mediated transformation
Journal name Molecular Biotechnology   Check publisher's open access policy
ISSN 1073-6085
1559-0305
Publication date 2008-09-01
Sub-type Article (original research)
DOI 10.1007/s12033-008-9065-4
Volume 40
Issue 1
Start page 77
End page 86
Total pages 10
Place of publication Totowa, NJ, United States
Publisher Humana Press
Language eng
Formatted abstract
Thlaspi caerulescens L. is well known as a Zn/Cd hyperaccumulator. The genetic manipulation of T. caerulescens through transgenic technology can modify plant features for use in phytoremediation. Here, we describe the efficient transformation of T. caerulescens using Agrobacterium tumefaciens strain EHA105 harboring a binary vector pBI121 with the nptII gene as a selectable marker, the gus gene as a reporter and a foreign catalase gene. Based on the optimal concentration of growth regulators, the shoot cluster regeneration system via callus phase provided the basis of the genetic transformation in T. caerulescens. The key variables in transformation were examined, such as co-cultivation period and bacterial suspension density. Optimizing factors for T-DNA delivery resulted in kanamycin-resistant transgenic shoots with transformation efficiency more than 20%, proven by histochemical GUS assay and PCR analysis. Southern analysis of nptII and RT-PCR of catalase gene demonstrated that the foreign genes were integrated in the genome of transformed plantlets. Moreover, the activity of catalase enzyme in transgenic plants was obviously higher than in wild-type plants. This method offers new prospects for the genetic engineering of this important hyperaccumulator species.
Keyword Agrobacterium tumefaciens
Thlaspi caerulescens
Genetic transformation
Hyperaccumulator
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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Created: Fri, 21 Oct 2011, 22:24:23 EST by Wei Wei on behalf of Queensland Brain Institute