BjDHNs confer heavy-metal tolerance in plants

Xu, Jin, Zhang, Yuxiu, Wei, Wei, Han, Lu, Guan, Ziqiu, Wang, Zi and Chai, Tuanyao (2008) BjDHNs confer heavy-metal tolerance in plants. Molecular Biotechnology, 38 2: 91-98. doi:10.1007/s12033-007-9005-8

Author Xu, Jin
Zhang, Yuxiu
Wei, Wei
Han, Lu
Guan, Ziqiu
Wang, Zi
Chai, Tuanyao
Title BjDHNs confer heavy-metal tolerance in plants
Formatted title
BjDHNs confer heavy-metal tolerance in plants
Journal name Molecular Biotechnology   Check publisher's open access policy
ISSN 1073-6085
Publication date 2008-02
Sub-type Article (original research)
DOI 10.1007/s12033-007-9005-8
Volume 38
Issue 2
Start page 91
End page 98
Total pages 8
Place of publication Totowa, NJ, United States
Publisher Humana Press
Language eng
Formatted abstract
Dehydrin gene transcript could be induced by heavy metals, and some dehydrins possess the ability to bind metals. However, the correlation between dehydrins and heavy-metal stress is unknown. In order to elucidate the contribution of dehydrins to heavy-metal stress tolerance in plants, we cloned two SK2-type dehydrin genes from heavy-metal hyperaccumulator Brassica juncea, and investigated their Cd/Zn tolerance in transgenic plants. Semi-quantitative RT-PCR analysis revealed that BjDHN2/BjDHN3 expressed in the leaves, stems and roots at a low level and were up-regulated by heavy metals. Antisense BjDHN3 Brassica juncea plants showed more electrolyte leakage and higher malondialdehyde production than the control plants when exposed to heavy metals, and the total amount of metals accumulated in the whole plant was reduced. Transgenic tobacco plants overexpressing BjDHN2/BjDHN3 showed lower electrolyte leakage and malondialdehyde production than the control plants when exposed to Cd/Zn. These results indicated that BjDHN2/BjDHN3 enhanced the tolerance for heavy metals by reducing lipid peroxidation and maintaining membrane stability in the plants.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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Created: Fri, 21 Oct 2011, 12:05:50 EST by Wei Wei on behalf of Queensland Brain Institute