Sequence polymorphisms in the flanking regions of microsatellite markers

Henry, R. J. and Ablett, G. (2008). Sequence polymorphisms in the flanking regions of microsatellite markers. In R. J. Henry (Ed.), Plant genotyping II: SNP technology (pp. 68-77) Wallingford, United Kingdom: CAB International. doi:10.1079/9781845933821.0068


Author Henry, R. J.
Ablett, G.
Title of chapter Sequence polymorphisms in the flanking regions of microsatellite markers
Title of book Plant genotyping II: SNP technology
Place of Publication Wallingford, United Kingdom
Publisher CAB International
Publication Year 2008
Sub-type Research book chapter (original research)
DOI 10.1079/9781845933821.0068
ISBN 9781845933821
1845933826
Editor R. J. Henry
Chapter number 4
Start page 68
End page 77
Total pages 10
Total chapters 15
Language eng
Abstract/Summary SNP markers may be discovered using many strategies. This chapter describes the approach of finding SNP markers in the flanking sequences surrounding microsatellites (simple sequence repeats (SSRs)). A major advantage of this strategy is that SSR markers have been widely studied and mapped in plant genomes, thus providing a readily available source of genetic loci that have already been mapped and that are readily convertible to SNP markers. SSRs have been markers of choice because they are highly informative, easy to use and many are now well-characterized. The topics covered include: SSR analysis; the main reasons for using SNP markers; the reason for using SSR markers to find SNPs; SNPs in wheat SSR markers; SNP discovery methods; pyrophosphate sequencing.
Keyword Bread wheat
Mutation
Loci
Ssr
Map
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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Created: Fri, 21 Oct 2011, 10:33:24 EST by Professor Robert Henry on behalf of Qld Alliance for Agriculture and Food Innovation