Transcriptional regulators of the GAD acid stress island are carried by effector protein-encoding prophages and indirectly control type III secretion in enterohemorrhagic Escherichia coli O157:H7

Tree, Jai J., Roe, Andrew J., Flockhart, Allen, McAteer, Sean P., Xu, Xuefang, Shaw, Darren, Mahajan, Arvind, Beatson, Scott A., Best, Angus, Lotz, Sabrina, Woodward, Martin J., La Ragione, Roberto, Murphy, Kenan C., Leong, John M. and Gally, David L. (2011) Transcriptional regulators of the GAD acid stress island are carried by effector protein-encoding prophages and indirectly control type III secretion in enterohemorrhagic Escherichia coli O157:H7. Molecular Microbiology, 80 5: 1349-1365. doi:10.1111/j.1365-2958.2011.07650.x


Author Tree, Jai J.
Roe, Andrew J.
Flockhart, Allen
McAteer, Sean P.
Xu, Xuefang
Shaw, Darren
Mahajan, Arvind
Beatson, Scott A.
Best, Angus
Lotz, Sabrina
Woodward, Martin J.
La Ragione, Roberto
Murphy, Kenan C.
Leong, John M.
Gally, David L.
Title Transcriptional regulators of the GAD acid stress island are carried by effector protein-encoding prophages and indirectly control type III secretion in enterohemorrhagic Escherichia coli O157:H7
Formatted title
Transcriptional regulators of the GAD acid stress island are carried by effector protein-encoding prophages and indirectly control type III secretion in enterohemorrhagic Escherichia coli O157:H7
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
1365-2958
Publication date 2011-06
Sub-type Article (original research)
DOI 10.1111/j.1365-2958.2011.07650.x
Volume 80
Issue 5
Start page 1349
End page 1365
Total pages 17
Place of publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
Type III secretion (T3S) plays a pivotal role in the colonization of ruminant hosts by Enterohemorrhagic Escherichia coli (EHEC). The T3S system translocates effector proteins into host cells to promote bacterial attachment and persistence. The repertoire and variation in prophage regions underpins differences in the pathogenesis and epidemiology of EHEC strains. In this study, we have used a collection of deletions in cryptic prophages and EHEC O157 O-islands to screen for novel regulators of T3S. Using this approach we have identified a family of homologous AraC-like regulators that indirectly repress T3S. These prophage-encoded secretion regulator genes (psr) are found exclusively on prophages and are associated with effector loci and the T3S activating Pch family of regulators. Transcriptional profiling, mutagenesis and DNA binding studies were used to show that these regulators usurp the conserved GAD acid stress resistance system to regulate T3S by increasing the expression of GadE (YhiE) and YhiF and that this regulation follows attachment to bovine epithelial cells. We further demonstrate that PsrA and effectors encoded within cryptic prophage CP933-N are required for persistence in a ruminant model of colonization.
Keyword H-NS
Genome sequence
Gene-expression
O157-H7
Evolution
Salmonella
Resistance
Virulence
K-12
Identification
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 26 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 21 Oct 2011, 03:18:33 EST by Dr Scott Beatson on behalf of School of Chemistry & Molecular Biosciences