An ELISA to detect serum antibodies to the salivary gland toxin of Ixodes holocyclus Neumann in dogs and rodents

Hall-Mendelin, S., O'Donoghue, P., Atwell, R., Lee, R. and Hall, R.A. (2011) An ELISA to detect serum antibodies to the salivary gland toxin of Ixodes holocyclus Neumann in dogs and rodents. Journal of Parasitological Research, 2011 : Article number 283416.


Author Hall-Mendelin, S.
O'Donoghue, P.
Atwell, R.
Lee, R.
Hall, R.A.
Title An ELISA to detect serum antibodies to the salivary gland toxin of Ixodes holocyclus Neumann in dogs and rodents
Journal name Journal of Parasitological Research   Check publisher's open access policy
Publication date 2011-05-18
Sub-type Article
DOI 10.1155/2011/283416
Volume number 2011
ISSN 2090-0023
Start page Article number 283416
Total pages 6
Place of publication United States
Publisher Hindawi Publishing Corporation
Collection year 2012
Language eng
Abstract The Ixodes holocyclus tick causes paralysis in up to 10,000 companion and domestic animals each year in Australia. Treatment requires the removal of the parasite and the administration of a commercial tick antiserum that is prepared from hyperimmune dogs. Each batch of this serum is initially tested for toxin-neutralising potency in a mouse bioassay that is expensive, time consuming, and subjective. With the aim of developing a rapid in vitro assay to replace the bioassay, we used a partially purified antigen prepared from I. holocyclus salivary glands to develop an ELISA to detect toxin-reactive antibodies in hyperimmune dog sera. The optimised ELISA reliably detected antibodies reactive to I. holocyclus salivary gland antigens. Parallel testing of sera with a negative control antigen prepared from the salivary glands of the nontoxic tick Rhipicephalus (Boophilus) microplus provided further evidence that we were detecting toxin-specific antibodies in the assay. Using the ELISA, we could also detect antibodies induced in rats after experimental infestation with I. holocyclus. This assay shows promise as an alternative means of assessing the potency of batches of hyperimmune dog serum and to screen for toxin-reactive monoclonal antibodies produced from immunised rodents.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 20 Oct 2011, 16:40:16 EST by Professor Peter O'donoghue on behalf of School of Chemistry & Molecular Biosciences