Xanthine dehydrogenase electrocatalysis : Autocatalysis and novel activity

Kalimuthu, Palraj, Leimkuhler, Silke and Bernhardt, Paul V. (2011) Xanthine dehydrogenase electrocatalysis : Autocatalysis and novel activity. Journal of Physical Chemistry B, 115 11: 2655-2662. doi:10.1021/jp111809f


Author Kalimuthu, Palraj
Leimkuhler, Silke
Bernhardt, Paul V.
Title Xanthine dehydrogenase electrocatalysis : Autocatalysis and novel activity
Journal name Journal of Physical Chemistry B   Check publisher's open access policy
ISSN 1520-6106
1089-5647
Publication date 2011-03-24
Sub-type Article (original research)
DOI 10.1021/jp111809f
Volume 115
Issue 11
Start page 2655
End page 2662
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2012
Language eng
Formatted abstract The enzyme xanthine dehydrogenase (XDH) from the purple photosynthetic bacterium Rhodobacter capsulatus catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid as part of purine metabolism. The native electron acceptor is NAD+ but herein we show that uric acid in its 2-electron oxidized form is able to act as an artificial electron acceptor from XDH in an electrochemically driven catalytic system. Hypoxanthine oxidation is also observed with the novel production of uric acid in a series of two consecutive 2-electron oxidation reactions via xanthine. XDH exhibits native activity in terms of its pH optimum and inhibition by allopurinol.
Keyword Pyrolytic-Graphite Electrodes
Uric-Acid Oxidation
Rhodobacter-Capsulatus
Electrochemical Oxidation
Glassy-Carbon
Cyclic Voltammetry
Crystal-Structures
Gold Electrode
Oxidase
Mechanism
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 42 Abstract Views  -  Detailed Statistics
Created: Thu, 20 Oct 2011, 16:17:14 EST by System User on behalf of School of Chemistry & Molecular Biosciences