Xanthine dehydrogenase electrocatalysis : Autocatalysis and novel activity

Kalimuthu, Palraj, Leimkuhler, Silke and Bernhardt, Paul V. (2011) Xanthine dehydrogenase electrocatalysis : Autocatalysis and novel activity. Journal of Physical Chemistry B, 115 11: 2655-2662. doi:10.1021/jp111809f

Author Kalimuthu, Palraj
Leimkuhler, Silke
Bernhardt, Paul V.
Title Xanthine dehydrogenase electrocatalysis : Autocatalysis and novel activity
Journal name Journal of Physical Chemistry B   Check publisher's open access policy
ISSN 1520-6106
Publication date 2011-03-24
Sub-type Article (original research)
DOI 10.1021/jp111809f
Volume 115
Issue 11
Start page 2655
End page 2662
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2012
Language eng
Formatted abstract
The enzyme xanthine dehydrogenase (XDH) from the purple photosynthetic bacterium Rhodobacter capsulatus catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid as part of purine metabolism. The native electron acceptor is NAD+ but herein we show that uric acid in its 2-electron oxidized form is able to act as an artificial electron acceptor from XDH in an electrochemically driven catalytic system. Hypoxanthine oxidation is also observed with the novel production of uric acid in a series of two consecutive 2-electron oxidation reactions via xanthine. XDH exhibits native activity in terms of its pH optimum and inhibition by allopurinol.
Keyword Pyrolytic-Graphite Electrodes
Uric-Acid Oxidation
Electrochemical Oxidation
Cyclic Voltammetry
Gold Electrode
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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