Molecular analysis of Mycobacterium tuberculosis causing multidrug-resistant tuberculosis meningitis

Caws, M., Thwaites, G. E., Duy, P. M., Tho, D. Q., Lan, N. T., Hoa, D. V., Chau, T. T., Huyen, M. N., Anh, P. T., Chau, N. V., Chinh, T. N., Stepniewska, K. and Farrar, J. (2007) Molecular analysis of Mycobacterium tuberculosis causing multidrug-resistant tuberculosis meningitis. International Journal of Tuberculosis and Lung Disease, 11 2: 202-208.

Author Caws, M.
Thwaites, G. E.
Duy, P. M.
Tho, D. Q.
Lan, N. T.
Hoa, D. V.
Chau, T. T.
Huyen, M. N.
Anh, P. T.
Chau, N. V.
Chinh, T. N.
Stepniewska, K.
Farrar, J.
Title Molecular analysis of Mycobacterium tuberculosis causing multidrug-resistant tuberculosis meningitis
Formatted title
Molecular analysis of Mycobacterium tuberculosis causing multidrug-resistant tuberculosis meningitis
Journal name International Journal of Tuberculosis and Lung Disease   Check publisher's open access policy
ISSN 1027-3719
1815-7920
Publication date 2007-02
Sub-type Article (original research)
Volume 11
Issue 2
Start page 202
End page 208
Total pages 7
Place of publication Paris, France
Publisher International Union against Tuberculosis and Lung Disease (IUATLD)
Language eng
Formatted abstract
SETTING: Tertiary referral hospitals in southern Vietnam.
OBJECTIVE: Molecular characterisation of multidrug-resistant (MDR) tuberculous meningitis (TBM).
DESIGN: Mycobacterium tuberculosis isolates from the cerebrospinal fluid (CSF) of 198 Vietnamese adults were compared with 237 isolates from patients with pulmonary tuberculosis (PTB) matched for age, sex and residential district. Isolates resistant to isoniazid or rifampicin (RMP) were sequenced in the rpoB and katG genes, inhA promoter and oxyR-ahpC intergenic regions.
RESULTS: While drug resistance rates were lower in the CSF (2.5% MDR) than pulmonary isolates (5.9% MDR), the difference was not significant. The most commonly mutated codons were 531, 526 and 516 in rpoB and 315 in katG. Four novel triple mutants in rpoB were identified.
CONCLUSION: RMP resistance is a good surrogate marker for MDR-TBM in this setting. However, probes directed against these three codons would have a maximum sensitivity of only 65%. A rapid phenotypic detection test may be more applicable for the diagnosis of MDR-TBM.
Keyword Tuberculosis
Multidrug-resistant
Meningitis
Vietnam
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 20 Oct 2011, 14:48:46 EST by Dr Minh Duy Phan on behalf of School of Chemistry & Molecular Biosciences