PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis

Caws, Maxine, Tho, Dau Quang, Duy, Phan Minh, Lan, Nguyen Thi Ngoc, Hoa, Dai Viet, Torok, Mili Estee, Chau, Tran Thi Hong, Chau, Nguyen Van Vinh, Chinh, Nguyen Tran and Farrar, Jeremy (2007) PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. Journal of Clinical Microbiology, 45 6: 1789-1793. doi:10.1128/JCM.01960-06

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Author Caws, Maxine
Tho, Dau Quang
Duy, Phan Minh
Lan, Nguyen Thi Ngoc
Hoa, Dai Viet
Torok, Mili Estee
Chau, Tran Thi Hong
Chau, Nguyen Van Vinh
Chinh, Nguyen Tran
Farrar, Jeremy
Title PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis
Formatted title
PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1070-633X
1098-660X
Publication date 2007-06
Sub-type Article (original research)
DOI 10.1128/JCM.01960-06
Open Access Status File (Publisher version)
Volume 45
Issue 6
Start page 1789
End page 1793
Total pages 5
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
PCR-restriction fragment length poymorphism (PCR-RFLP) is a simple, robust technique for the rapid identification of isoniazid-resistant Mycobacterium tuberculosis. One hundred consecutive isolates from a Vietnamese tuberculosis hospital were tested by MspA1I PCR-RFLP for the detection of isoniazid-resistant katG_315 mutants. The test had a sensitivity of 80% and a specificity of 100% against conventional phenotypic drug susceptibility testing. The positive and negative predictive values were 1 and 0.86, respectively. None of the discrepant isolates had mutant katG_315 codons by sequencing. The test is cheap (less than $1.50 per test), specific, and suitable for the rapid identification of isoniazid resistance in regions with a high prevalence of katG_315 mutants among isoniazid-resistant M. tuberculosis isolates.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 20 Oct 2011, 14:45:11 EST by Dr Minh Duy Phan on behalf of School of Chemistry & Molecular Biosciences