Glycoside hydrolase activities of thermophilic bacterial consortia adapted to switchgrass

Gladden, John M., Allgaier, Martin, Miller, Christopher S., Hazen, Terry C., VanderGheynst, Jean S., Hugenholtz, Philip, Simmons, Blake A. and Singer, Steven W. (2011) Glycoside hydrolase activities of thermophilic bacterial consortia adapted to switchgrass. Applied and Environmental Microbiology, 77 16: 5804-5812. doi:10.1128/AEM.00032-11

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Author Gladden, John M.
Allgaier, Martin
Miller, Christopher S.
Hazen, Terry C.
VanderGheynst, Jean S.
Hugenholtz, Philip
Simmons, Blake A.
Singer, Steven W.
Title Glycoside hydrolase activities of thermophilic bacterial consortia adapted to switchgrass
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2011-08-15
Sub-type Article (original research)
DOI 10.1128/AEM.00032-11
Open Access Status File (Publisher version)
Volume 77
Issue 16
Start page 5804
End page 5812
Total pages 9
Place of publication United States
Publisher American Society for Microbiology
Collection year 2012
Language eng
Abstract Industrial-scale biofuel production requires robust enzymatic cocktails to produce fermentable sugars from lignocellulosic biomass. Thermophilic bacterial consortia are a potential source of cellulases and hemicellulases adapted to harsher reaction conditions than commercial fungal enzymes. Compost-derived microbial consortia were adapted to switchgrass at 60°C to develop thermophilic biomass-degrading consortia for detailed studies. Microbial community analysis using small-subunit rRNA gene amplicon pyrosequencing and short-read metagenomic sequencing demonstrated that thermophilic adaptation to switchgrass resulted in low-diversity bacterial consortia with a high abundance of bacteria related to thermophilic paenibacilli, Rhodothermus marinus, and Thermus thermophilus. At lower abundance, thermophilic Chloroflexi and an uncultivated lineage of the Gemmatimonadetes phylum were observed. Supernatants isolated from these consortia had high levels of xylanase and endoglucanase activities. Compared to commercial enzyme preparations, the endoglucanase enzymes had a higher thermotolerance and were more stable in the presence of 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]), an ionic liquid used for biomass pretreatment. The supernatants were used to saccharify [C2mim][OAc]-pretreated switchgrass at elevated temperatures (up to 80°C), demonstrating that these consortia are an excellent source of enzymes for the development of enzymatic cocktails tailored to more extreme reaction conditions.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Supplemental material (read only) - http://aem.asm.org/content/77/16/5804/suppl/DC1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 20 Oct 2011, 09:48:29 EST by Professor Philip Hugenholtz on behalf of School of Chemistry & Molecular Biosciences