Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes

Ng, Terry Fei Fan, Willner, Dana L., Lim, Yan Wei, Schmieder, Robert, Chau, Betty, Nilsson, Christina, Anthony, Simon, Ruan, Yijun, Rohwer, Forest and Breitbart, Mya (2011) Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes. PLoS One, 6 6: Article number e20579. doi:10.1371/journal.pone.0020579


Author Ng, Terry Fei Fan
Willner, Dana L.
Lim, Yan Wei
Schmieder, Robert
Chau, Betty
Nilsson, Christina
Anthony, Simon
Ruan, Yijun
Rohwer, Forest
Breitbart, Mya
Title Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-06-06
Sub-type Article (original research)
DOI 10.1371/journal.pone.0020579
Open Access Status DOI
Volume 6
Issue 6
Start page Article number e20579
Total pages 10
Place of publication United States
Publisher Public Library of Science
Collection year 2012
Language eng
Abstract Viruses are the most abundant and diverse genetic entities on Earth; however, broad surveys of viral diversity are hindered by the lack of a universal assay for viruses and the inability to sample a sufficient number of individual hosts. This study utilized vector-enabled metagenomics (VEM) to provide a snapshot of the diversity of DNA viruses present in three mosquito samples from San Diego, California. The majority of the sequences were novel, suggesting that the viral community in mosquitoes, as well as the animal and plant hosts they feed on, is highly diverse and largely uncharacterized. Each mosquito sample contained a distinct viral community. The mosquito viromes contained sequences related to a broad range of animal, plant, insect and bacterial viruses. Animal viruses identified included anelloviruses, circoviruses, herpesviruses, poxviruses, and papillomaviruses, which mosquitoes may have obtained from vertebrate hosts during blood feeding. Notably, sequences related to human papillomaviruses were identified in one of the mosquito samples. Sequences similar to plant viruses were identified in all mosquito viromes, which were potentially acquired through feeding on plant nectar. Numerous bacteriophages and insect viruses were also detected, including a novel densovirus likely infecting Culex erythrothorax. Through sampling insect vectors, VEM enables broad survey of viral diversity and has significantly increased our knowledge of the DNA viruses present in mosquitoes.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 91 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 96 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 20 Oct 2011, 09:01:42 EST by Dana Willner on behalf of School of Chemistry & Molecular Biosciences